| 茯苓菌丝体多糖径向流色谱分离参数优化 |
| |
| 引用本文： | 魏海龙,贺亮,甘庆军,胡传久,吴学谦,程俊文,李海波,付立忠.茯苓菌丝体多糖径向流色谱分离参数优化[J].浙江林业科技,2011(2). |
| |
| 作者姓名： | 魏海龙 贺亮 甘庆军 胡传久 吴学谦 程俊文 李海波 付立忠 |
| |
| 作者单位： | 浙江省林业科学研究院浙江省森林资源生物与化学利用重点实验室;丽水市食用菌研究开发中心;庆元县食用菌科研中心; |
| |
| 基金项目： | 国家林业局948项目“食药用菌活性多糖生物酶法提取与检测技术引进”(2008-4-64); 浙江省林业科技项目(08A07); 浙江省省院合作项目(2008SY02) |
| |
| 摘 要： | 在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化茯苓菌丝体多糖径向流色谱分离的工艺条件。响应面分析结果表明,当样品浓度为8 mg/mL,上样量为40 mL,上样流速为25 mL/min,洗脱流速为33 mL/min时,多糖回收率达到83.26%,比传统轴向色谱分离及化学方法效率更高,时间更短。
|
| 关 键 词： | 茯苓菌丝体多糖 径向流色谱 响应面法 分离 |
Separation of Polysaccharides by Radial Flow Ion-exchange from Poria cocos |
| |
| WEI Hai-long,HE Liang,GAN Qing-jun,HU Chuan-jiu,WU Xue-qian,CHENG Jun-wen,LI Hai-bo,FU Li-zhong.Separation of Polysaccharides by Radial Flow Ion-exchange from Poria cocos[J].Journal of Zhejiang Forestry Science and Technology,2011(2). |
| |
| Authors: | WEI Hai-long HE Liang GAN Qing-jun HU Chuan-jiu WU Xue-qian CHENG Jun-wen LI Hai-bo FU Li-zhong |
| |
| Institution: | WEI Hai-long1,HE Liang1,2,GAN Qing-jun3,HU Chuan-jiu1,WU Xue-qian1,CHENG Jun-wen1,LI Hai-bo1,FU Li-zhong1,2(1.Key Laboratory of Biol-chemical Utilization of Forest Resources,Zhejiang Forestry Academy,Hangzhou 310023,China,2.Lishui Edible Fungi R & D Center of Zhejiang,Lishui 323000,3.Qingyuan Edible Fungi R & D Center of Zhejiang,Qingyuan 323800,China) |
| |
| Abstract: | Seperation technology was tested by radial flow chromatography(RFC) packed with ion-exchange.Based on the single factor test,response surface method(RSM) was employed to obtain the best result.The purification efficiency of RFC was compared with that of axial column and traditional chemical method.RSM analysis showed that the optimal conditions for the purification of Poria cocos polysaccharides with RFC were as follows: sample concentration of 8 mg/mL,sample volume of 40 mL,sample flow-rate of 25 mL/min an... |
| |
| Keywords: | Poria cocos polysaccharides radial flow chromatography RSM separation |
| 本文献已被 CNKI 等数据库收录！ |
|
