| 猪圆环病毒2型ORF2基因的定点突变及其真核表达载体的构建 |
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| 引用本文： | 张艳萍,苏丹萍,贺东生.猪圆环病毒2型ORF2基因的定点突变及其真核表达载体的构建[J].中国畜牧兽医,2011,38(2). |
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| 作者姓名： | 张艳萍 苏丹萍 贺东生 |
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| 作者单位： | 华南农业大学兽医学院广东省人畜共患病重点实验室,广东广州,510642 |
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| 摘 要： | 根据GenBank发表的猪圆环病毒2型(PCV2)ORF2基因序列设计并合成引物,采用重叠延伸PCR(splice overlap extension PCR,SOE-PCR)定点诱变技术对ORF2基因进行定点突变。DNA测序结果表明,ORF2基因片段的372—378 bp处的碱基由TCTAGAT突变为ATTGGAT,从而破坏酶切位点XbaⅠ,成功获得ORF2的突变基因片段。进而将其连接到腺病毒真核表达载体pAD5-Blue中,成功构建真核表达载体,并获得PCV2的Cap蛋白表达产物,为PCV2新型疫苗研究奠定了重要基础。
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| 关 键 词： | 猪圆环病毒2型 ORF2基因 重叠延伸PCR pAD5-Blue Cap蛋白 |
Site-directed Mutagenesis of PCV2 ORF2 Gene Based on Overlap Extension PCR and Construction of Eukaryotic Expression Vector |
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| ZHANG Yan-ping,SU Dan-ping,HE Dong-sheng.Site-directed Mutagenesis of PCV2 ORF2 Gene Based on Overlap Extension PCR and Construction of Eukaryotic Expression Vector[J].China Animal Husbandry & Veterinary Medicine,2011,38(2). |
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| Authors: | ZHANG Yan-ping SU Dan-ping HE Dong-sheng |
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| Institution: | ZHANG Yan-ping,SU Dan-ping,HE Dong-sheng(Guangdong Key Laboratory of Zoonosis,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | |
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| Keywords: | PCV2 ORF2 gene overlap extension PCR pAD5-Blue protein of Cap |
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