| 谷氨酰胺、氯化镧对石斛兰生根及POD表达的影响 |
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| 引用本文： | 刘娟娟.谷氨酰胺、氯化镧对石斛兰生根及POD表达的影响[J].安徽农学通报,2010,16(19):47-49. |
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| 作者姓名： | 刘娟娟 |
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| 作者单位： | 阜阳市创业水务有限公司,安徽阜阳,236000 |
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| 摘 要： | 采用组织培养方法,探讨了谷氨酰胺及氯化镧对石斛兰生根及POD表达的影响。结果表明：谷氨酰胺、氯化镧对石斛兰生根数及生长状况的影响与处理的浓度有关。随着谷氨酰胺浓度的增加,石斛兰总生根数、平均生根数呈下降趋势,当处理30d后,添加2mg/L谷氨酰胺会产生最大的平均生根数,为5.78根。氯化镧的浓度为0.05～50mg/L时对石斛兰生根及生长起促进作用,且组培苗生长状况要优于谷氨酰胺处理。在同工酶表达上,相对谷氨酰胺处理,氯化镧处理后的石斛兰POD同工酶在活性上要更强,在表达类型类型上要更丰富。
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| 关 键 词： | 石斛兰 谷氨酰胺 氯化镧 同工酶 |
Effect of Glutamine,Lanthanum Chloride on the Rooting and POD Expression of Dendrobium |
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| Li Juanjuan.Effect of Glutamine,Lanthanum Chloride on the Rooting and POD Expression of Dendrobium[J].Auhui Agricultural Science Bulletin,2010,16(19):47-49. |
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| Authors: | Li Juanjuan |
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| Institution: | Li Juanjuan(Fuyang Entrepreneurial water Co.,LTD,Fuyang 236000,China) |
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| Abstract: | Tissue culture method was used to explore the glutamine and lanthanum chloride on the rooting and POD expression of Dendrobium.The results showed that,influence of glutamine,lanthanum chloride on the rooting and growth of Dendrobium was related to the treatment concentration.With the increasing of glutamine concentration,the total rooting number and the average rooting number appeared to drop;After treating for 30 days,when the glutamine contentration reached 2mg/L,the maximum rooting number was 5.78.when t... |
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| Keywords: | Dendrobium Glutamine Lanthanum chloride Isozyme |
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