| 玉米幼胚愈伤组织的诱导和植株再生的QTL分析 |
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| 引用本文： | 张红伟,刘亚娟,郭晓琳,张峰,李建生,陈刚,孙东发,谭振波.玉米幼胚愈伤组织的诱导和植株再生的QTL分析[J].作物学报,2006,32(3):385-389. |
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| 作者姓名： | 张红伟 刘亚娟 郭晓琳 张峰 李建生 陈刚 孙东发 谭振波 |
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| 作者单位： | 北京大北农农业科技研究院，北京 100085 |
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| 摘 要： | 以黄早四和Mo17为亲本组配的239个RIL群体，构建了101个SSR标记的遗传图谱，覆盖玉米基因组1 422.7 cM，标记间的平均距离为15.6 cM。以玉米幼胚为外植体、改良N6为基本培养基，对亲本及RIL群体的组培性状进行了评价。采用复合区间作图法在第2、3、5、6、8和9染色体上定位了控制出愈率、Ⅱ型愈伤组织诱导率、绿点及绿苗分化率的8个QTL，并对其遗传效应进行了分析，其基因加性效应能解释相应性状表型方差的4.78%~14.02%。
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| 关 键 词： | 玉米 幼胚 植株再生 QTL |
| 收稿时间： | 2005-01-18 |
| 修稿时间： | 2005-08-01 |
QTL Mapping for Callus Induction and Plant Regeneration in Maize Immature Embryos |
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| ZHANG Hong-Wei,LIU Ya-Juan,GUO Xiao-Lin,ZHANG Feng,LI Jian-Sheng,CHEN Gang,SUN Dong-Fa,TAN Zhen-Bo.QTL Mapping for Callus Induction and Plant Regeneration in Maize Immature Embryos[J].Acta Agronomica Sinica,2006,32(3):385-389. |
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| Authors: | ZHANG Hong-Wei LIU Ya-Juan GUO Xiao-Lin ZHANG Feng LI Jian-Sheng CHEN Gang SUN Dong-Fa TAN Zhen-Bo |
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| Institution: | Beijing DBN Agricultural Sci & Tech Research Institute, Beijing 100085 |
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| Abstract: | Inbred lines Huangzao4 and Mo17 were used to develop a population consisted of 239 Recombinant inbred lines（RILs）. Based on the F8 generation population, a SSR linkage map was constructed with 101 SSR loci, covering maize genome 1 422.7 cM with an average interval length of 15.6 cM. The 239 RILs and their parental lines were evaluated for tissue cultural traits，including calli initiation frequency, type Ⅱ calli initiation frequency, green buds initiation frequency and plant regeneration frequency. With the method of composite interval mapping, 8 QTLs were identified and located on chromosome 2, 3, 5, 6, 8 and 9. 5 QTLs of regeneration frequency were identified on chromosome 2, 3, 5, 6, and 9, which can explained 6.29%, 5.19%, 7.88%, 14.02% and 10.4% of the phenotypic variance, respectively. 1 QTL on chromosome 5, linked with markers umc1966 was identified with minor effects conferring type Ⅱ calli initiation frequency, which only explained 6.60% of the phenotypic variance. 2 QTLs on chromosome 8, linked with markers bnlg2235 and bnlg240, were identified controlling calli initiation frequency and green buds initiation frequency, which explained 9.51% and 4.78% of the phenotypic variance, respectively. Two QTLs controlling type Ⅱ callus rate and plant regeneration rate were located in the neighboring region of chromsome 8, and these two traits were correlated between each other. However, further study is needed to make clear if the correlation is due to one pleiotropic gene or the linkage of two genes. |
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| Keywords: | QTL |
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