| 美国白蛾SSR反应体系的优化与初步应用 |
| |
| 引用本文： | 杜娟,南宫自艳,周国娜,高宝嘉.美国白蛾SSR反应体系的优化与初步应用[J].林业科学,2010,46(3). |
| |
| 作者姓名： | 杜娟 南宫自艳 周国娜 高宝嘉 |
| |
| 作者单位： | 1. 河北农业大学林学院,保定,071000
2. 河北农业大学植物保护学院,保定,071000 |
| |
| 基金项目： | 国家自然科学基金(30771739) |
| |
| 摘 要： | 我国于1979年首次在辽宁省丹东市发现美国白蛾(Hyphantria cunea)后,害虫扩散蔓延到山东、陕西、河北、天津和上海。美国白蛾繁殖量大,寄主植物种类繁多,扩散速度快,暴发时常食尽叶片。近
|
| 关 键 词： | 美国白蛾 SSR 反应体系 |
Optimization and Preliminary Application of SSR Analysis System in Hyphantria cunea |
| |
| Du Juan Nangong Ziyan Zhou Guona Gao Baojia.Optimization and Preliminary Application of SSR Analysis System in Hyphantria cunea[J].Scientia Silvae Sinicae,2010,46(3). |
| |
| Authors: | Du Juan Nangong Ziyan Zhou Guona Gao Baojia |
| |
| Institution: | 1. College of Forestry/a>;Agricultural University of Hebei Baoding 071000/a>;2. College of Plant Protection/a>;Agricultural University of Hebei Baoding 071000 |
| |
| Abstract: | An optimization SSR reaction system was established to investigate the genetic diversity of Hyphantria cunea (Drury). A population of Hyphantria cunea in Cangzhou was used to optimize PCR conditions in the SSR system. The results showed that the best condition was 0.40 mmol·L~(-1) dNTP,2.5 mmol·L~(-1) Mg~(2+), 0.32 μmol·L~(-1) primers, 1U Taq polymerase, 30 ～ 60 ng template DNA in 25 μL SSR reaction system and the optimal annealing temperature of primers was 52 ℃. Genetic diversity of five Hyphantria cunea populations was studied with this SSR reaction system, and the result showed the microsatellite primers were high polymorphic and this system was suitable and stable. |
| |
| Keywords: | Hyphantria cunea SSR analysis system |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
