| 不同比例红蓝光及光照强度对金线莲生理及叶绿素荧光特性的影响 |
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| 引用本文： | 潘可可,王克磊,李斌奇,张曦文,陈发兴.不同比例红蓝光及光照强度对金线莲生理及叶绿素荧光特性的影响[J].热带作物学报,2022,43(8):1628-1635. |
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| 作者姓名： | 潘可可 王克磊 李斌奇 张曦文 陈发兴 |
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| 作者单位： | 1.温州市农业科学研究院/浙南作物育种重点实验室,浙江温州 3250142.福建农林大学园艺学院,福建福州 3500023.福建农林大学机电工程学院,福建福州 350002 |
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| 基金项目： | 福建省发改委农业五新项目(B0310008) |
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| 摘 要： | 为探讨LED光源在金线莲工厂化栽培中的应用,利用研发的具有不同比例红（R）、蓝光（B）的LED灯作为光源,以白色荧光灯为对照（CK）,设置以下5个试验处理,T1：R/B(3/7), PPFD 20 μmol/(m2·s);T2：R/B(7/3), PPFD 20 μmol/(m2·s);T3：R/B(3/7), PPFD 30 μmol/(m2·s);T4：R/B(7/3), PPFD 30 μmol/(m2·s);T5：R/B(7/3), PPFD 50 μmol/(m2·s),研究不同比例红蓝光源及其光照强度对金线莲的生长、光合作用、叶绿素荧光反应和生理特性的影响。结果表明：与对照处理相比,T4、T5处理的金线莲株高、茎粗、植株干鲜重显著提高;T5处理的金线莲叶片净光合速率显著高于其他处理,而T1、T3处理间差异不显著,但显著高于T2、T4处理。不同比例红蓝光处理下金线莲叶片的叶绿素含量显著高于对照处理,在相同的光照强度下,R/B(7/3)处理的金线莲叶片叶绿素含量大于R/B(3/7)处理。不同比例红蓝光源处理的金线莲叶片最大光化学效率、光系统II活性均显著高于对照处理;红光比例减少,蓝光比例增加,可降低金线莲叶片最大光化学效率、光系统II活性,同时光系统II实际光化学效率和光合电子传递效率也随之降低。T2处理可有效提高金线莲叶片SOD和CAT活性,但POD活性降低;T4处理下金线莲叶片POD和CAT活性降低,SOD活性升高;与对照相比,T1处理的金线莲叶片MDA含量显著增加,较对照提高19.4%,而T5处理与对照差异不显著。综合各处理金线莲生长来看,T5处理R/B(7/3), PPFD 50 μmol/(m2·s)的金线莲生长最好,保持较高的光合速率及生理活性。
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| 关 键 词： | 红蓝光比例 金线莲 生长 生理变化 叶绿素荧光 |
| 收稿时间： | 2021-12-13 |
Effects of Different Proportion of Red and Blue Light and Light Intensity on Physiology and Chlorophyll Fluorescence Characteristics of Anoectochilus roxburghii |
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| PAN Keke,WANG Kelei,LI Binqi,ZHANG Xiwen,CHEN Faxing.Effects of Different Proportion of Red and Blue Light and Light Intensity on Physiology and Chlorophyll Fluorescence Characteristics of Anoectochilus roxburghii[J].Chinese Journal of Tropical Crops,2022,43(8):1628-1635. |
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| Authors: | PAN Keke WANG Kelei LI Binqi ZHANG Xiwen CHEN Faxing |
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| Institution: | 1. Wenzhou Academy of Agricultural Sciences / Southern Zhejiang Key Laboratory of Crop Breeding, Wenzhou, Zhejiang 325014, China2. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China3. College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | In order to explore the application of LED lamp in the industrialized cultivation of Anoectochilus roxburghii, the LED lamp with different proportion of red and blue light was used as the light source. The white fluorescent lamp was used as the control (CK), with R/B(3/7), PPFD 20 μmol/(m2·s)(T1), R/B(7/3), PPFD 20 μmol/(m2·s)(T2), R/B(3/7), PPFD 30 μmol/(m2·s)(T3), R/B(7/3), PPFD 30 μmol/(m2·s)(T4), R/B(7/3), PPFD 50 μmol/(m2·s)(T5) five treatments. The effects of different ratios of red and blue light and light intensity on the growth, photosynthesis, chlorophyll fluorescence and physiological characteristics of A. roxburghii were studied. The results showed that plant height, stem diameter and dry and fresh weight of A. roxburghii treated with T4 and T5 were significantly higher than those treated with CK. Pn of A. roxburghii leaves in T5 was significantly higher than that in the other treatments, there was no significant difference between T1 and T3, but it was significantly higher than that in T2 and T4. Under different proportion of red and blue light treatment, the chlorophyll content of A. roxburghii leaves was significantly higher than that of CK. Under the same light intensity, the chlorophyll content of A. roxburghii leaves treated with R/B(7/3) was higher than that treated with R/B(3/7). Fv/Fm and Fv/Fo of A. roxburghii leaves treated with different red blue ratio light were significantly higher than those treated with CK. The decrease of red light ratio and the increase of blue light ratio could reduce Fv/Fm and Fv/Fo, and ΦPSII and ETR also decreased. T2 could effectively improve the activities of SOD and CAT, but the activity of POD decreased. The activities of POD and CAT decreased and SOD increased under T4. Compared with CK, the MDA content of A. roxburghii leaves in T1 increased significantly, 19.4% higher than CK, while there was no significant difference between T5 and CK. According to the growth of A. roxburghii, the treatment with R/B(7/3), PPFD 50 μmol/(m2·s) (T5) grew best and maintained high photosynthetic rate and physiological activity. |
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| Keywords: | red-to-blue light ratio Anoectochilus roxburghii growth physiology chlorophyll fluorescence |
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