| 用60Co辐射处理胰酶配制消化液的应用效果观察 |
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| 引用本文： | 丁建民,孙洪丽.用60Co辐射处理胰酶配制消化液的应用效果观察[J].中国兽药杂志,2004,38(9):50-51. |
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| 作者姓名： | 丁建民 孙洪丽 |
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| 作者单位： | 辽宁省益康生物制品厂,辽宁,辽阳,111000 |
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| 摘 要： | 应用60Co辐射灭菌法配制的胰酶溶液与常规过滤法配制的胰酶溶液消化犊牛睾丸组织和原代睾丸细胞,通过对比消化时间和获得的细胞数量,证实以200万拉德(rad)辐射处理的胰酶灭菌效果可靠,胰酶的消化活性不受影响.
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| 关 键 词： | 胰蛋白酶 辐射 灭菌 活力 |
| 文章编号： | 1002-1280(2004)09-0050-02 |
| 修稿时间： | 2004年4月2日 |
The application effects of the digestive solution prepared with trypsin sterilized by eradiating with 60Co |
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| DING Jian-min,SUN Hong-li.The application effects of the digestive solution prepared with trypsin sterilized by eradiating with 60Co[J].Chinese Journal of Veterinary Drug,2004,38(9):50-51. |
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| Authors: | DING Jian-min SUN Hong-li |
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| Abstract: | The trypsin was sterilized by eradiating with~(60)Co or by filtration and then trypsin working solution was prepared. The calf testicle cell culture and these primary cells culture were digested with this two kinds of trypsin solutions. The results showed that the disinfect effects of the trypsin sterilized with 2 million rad of eradiation with~(60)Co was credible, and the activity of the trypsin was not affected. |
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| Keywords: | trypsin eradiate sterilize |
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