Effects of nucleus,cytoplasm and male sterile nucleus-cytoplasm combinations on callus initiation in anther culture of wheat |
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| Authors: | P W Becraft G A Taylor |
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| Institution: | (1) Plant and Soil Science Dept., Montana State University, 59717 Bozeman, MT, USA;(2) Present address: Genetics Department, University of California, 94720 Berkeley, CA, USA |
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| Abstract: | Summary Nuclear and cytoplasmic factors affect tissue culture response in wheat (Triticum aestivum), and cytoplasmic male sterility may enhance callus initiation in anther culture. Three wheat nuclear genotypes, each in normal and two alien cytoplasms conferring cytoplasmic male sterility, were evaluated for callus initiation frequency in anther culture. Nuclear genotype had the greatest effect on callus initiation, but cytoplasm and nucleus X cytoplasm interaction also produced significant effects. The nuclear genotype of  Chris outperformed Butte and Coteau in all cytoplasms. Ordinary wheat and Triticum timopheevi cytoplasms outperformed Aegilops speltoides cytoplasm. Cytoplasmic male sterility did not increase callus initiation. This suggests manipulating the nuclear genotype is the best strategy for improving the capacity of wheat to initiate callus in anther culture. |
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| Keywords: | Triticum aestivum wheat anther culture callus initiation cytoplasm cytoplasmic male sterility |
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