| 野西瓜苗无性系建立的研究 |
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| 引用本文： | 郑亮,白玥,曲杨乐,吴君,姜长阳.野西瓜苗无性系建立的研究[J].辽宁农业科学,2009(3):5-8. |
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| 作者姓名： | 郑亮 白玥 曲杨乐 吴君 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁,大连,116029 |
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| 摘 要： | 以野西瓜苗嫩茎为材料,进行了愈伤组织诱导,愈伤组织和不定芽分化,试管苗生根,试管苗移栽、扦插和移植的研究,成功的建立起野西瓜苗的无性系。结果证明：MS＋BA 0.5 mg/L＋2,4-D 1.0-1.5 mg/L是野西瓜苗嫩茎愈伤组织诱导的理想培养基;MS＋AgNO31.0 mg/L＋BA 1.0 mg/L＋NAA 0.2-0.4 mg/L是诱导野西瓜苗嫩茎愈伤组织和不定芽分化的理想培养基;1/2 MS＋IAA 0.4 mg/L＋NAA 0.2 mg/L是诱导野西瓜苗试管苗生根培养的理想培养基;移植到山坡上的试管苗保持了野西瓜苗的生物性状,生长旺盛。
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| 关 键 词： | 野西瓜苗 愈伤组织 无性系 快速繁殖 |
Establishment of Asexual Line of Hibiscus trionum |
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| ZHENG Liang,BAI Yue,QU Yang-Le,WU Jun,JIANG Chang-Yang.Establishment of Asexual Line of Hibiscus trionum[J].Liaoning Agricultural Sciences,2009(3):5-8. |
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| Authors: | ZHENG Liang BAI Yue QU Yang-Le WU Jun JIANG Chang-Yang |
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| Institution: | ( College of Life Sciences, Liaoning Normal University, Dalian, Liapning 116029) |
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| Abstract: | The asexual line of Hibiscus trionum was established by the studies on the development of growing point, differentiation of adventitious buds, rooti of Hibiscus trionum. The results showed that the ng, cutting and transplantation of tube seedling for the sprouts ideal media for callus induction is MS ＋ BA 0.5 mg/L ＋ 2,4- D 1.0 - 1.5 mg/L. The best media for differentiation is MS ＋ AgNO3 1.0 mg/L ＋ BA 1.0 mg/L ＋ NAA 0.2 -0.4 mg/L. The medium of 1/2 MS ＋ IAA 0.4 mg/L ＋ NAA 0.2 mg/L is suitable for rooting and rhizome growth of tube seeding. The cinder is the ideal medium of transplanting and cutting. Field planting showes that the tube seeding grew well and keep all biological character. |
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| Keywords: | Hibiscus trionum Callus Clone Rapid propagation |
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