| 添加光合细菌对糙海参幼苗培育阶段的影响研究 |
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| 引用本文： | 姜松,王军红,范嗣刚,温为庚,黄桂菊,刘宝锁,张博,喻达辉.添加光合细菌对糙海参幼苗培育阶段的影响研究[J].南方水产,2014(6):78-82. |
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| 作者姓名： | 姜松 王军红 范嗣刚 温为庚 黄桂菊 刘宝锁 张博 喻达辉 |
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| 作者单位： | 1. 中国水产科学研究院南海水产研究所，农业部南海渔业资源开发利用重点实验室，广东 广州510300
2. 中国长江三峡集团中华鲟研究所,湖北 宜昌,443100 |
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| 基金项目： | 国家自然科学基金项目(31372525)；广东省海洋经济创新发展区域示范专项(GD2012-A01-002)；广东省海洋渔业科技推广专项项目 |
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| 摘 要： | 以受精后24h孵化出的糙海参（Holothuria scabra）耳状幼体为研究对象,探讨养殖水体不同量光合细菌对糙海参苗期生长、成活、消化道消化酶活性和养殖水体水质变化的影响。试验设4个处理,光合细菌（浓度为1×10^11cfu·mL^-1）添加量分别是0m L（组1,对照组）、50m L（组2）、100m L（组3）和150m L（组4）,每个处理设3个重复,每个重复放养4×10^4尾幼体于室内水泥池（5m×3 m×1.5m）中,试验周期为41 d。结果表明,添加光合细菌组的糙海参出苗体质量和成活率均显著高于对照组（P〈0.05）,而组3和组4的体质量和成活率又明显优于组2（P〈0.05）,组3和组4之间的差异不显著（P〉0.05）。添加光合细菌还能不同程度地影响糙海参苗体消化道蛋白酶、淀粉酶和纤维素酶的活性,试验组3种酶的活性均显著高于对照组（P〈0.05）,而组3和组4的蛋白酶活性和纤维素酶活性显著高于组2（P〈0.05）,淀粉酶活性在3个试验组之间无显著差异（P〉0.05）。在试验第10天后,各试验组氨氮（NH3-N）和亚硝酸盐含量显著低于对照组（P〈0.05）,在试验第20天后,各试验组化学需氧量（COD）的值显著低于对照组（P〈0.05）,而总磷（TP）的差异不大（P〉0.05）。表明光合细菌可以促进糙海参幼体的生长,提高消化酶活性和成活率,并改良育苗水体水质。
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| 关 键 词： | 糙海参 光合细菌 体质量 成活率 消化酶活性 水质 |
Influence of photosynthetic bacteria on breeding of Holothuria scabra larvae |
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| JIANG Song,WANG Junhong,FAN Sigang,WEN Weigeng,HUANG Guiju,LIU Baosuo,ZHANG Bo,YU Dahui.Influence of photosynthetic bacteria on breeding of Holothuria scabra larvae[J].South China Fisheries Science,2014(6):78-82. |
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| Authors: | JIANG Song WANG Junhong FAN Sigang WEN Weigeng HUANG Guiju LIU Baosuo ZHANG Bo YU Dahui |
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| Institution: | JIANG Song,WANG Junhong,FAN Sigang,WEN Weigeng,HUANG Guiju,LIU Baosuo,ZHANG Bo,YU Dahui(1.Key Lab. of South China Sea Fishery Resources Exploitation ＆ Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. Research Institute of Chinese Sturgeon, China Three Gorges Corporation, Yichang 443100, China) |
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| Abstract: | Taking Holothuria scabra auricularia larvae hatched after 24h fertilization as objects,we studied the influence of photosynthetic bacteria on their growth,survival and digestive enzymatic activities as well as on water quality. The experiments lasted 41 d and four groups were set up,each with three replicates. Photosynthetic bacteria（ 1 × 10^11cfu·mL^-1） of 0m L（ Group 1,control）,50m L（ Group 2）,100m L（ Group 3） and 150m L（ Group 4） were added respectively into tanks（ 5m × 3 m × 1. 5m） once every 5 d.Each tank was added with 4 × 10^4 larvae. The results show that the body weight and survival rate in the treatment groups（ Group 2,Group 3 and Group 4） were significantly higher than those in the control group（ P〈0. 05）,while the body weight and survival rate in Group 3 and Group 4 were significantly higher than those in Group 2（ P〈0. 05）,but there was no significant difference betweenGroup 3 and Group 4（ P〉0. 05）. Photosynthetic bacteria also affected the activities of protease,amylase and cellulose in intestines of larvae which in the treatment groups were significantly higher than those in the control group（ P〈0. 05）. The enzyme activities of protease and cellulose in Group 3 and Group 4 were significantly higher than those in Group 2（ P〈0. 05）,but there was no significant difference in enzyme activity of amylase among treatment groups（ P〉0. 05）. The ammonia nitrogen and nitrite contents in treatment groups were significantly lower than those in the control group（ P〈0. 05） after 10 d; the chemical oxygen demand（ COD） in treatment groups was significantly lower than that in control group（ P〈0. 05） after 20 d but no significant difference in total phosphorus（ TP）. The results indicate that photosynthetic bacteria can accelerate the growth,increase the survival rate and digestive enzymes activities of H. scabra larvae and improve aquaculture water quality. |
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| Keywords: | Holothuria scabra photosynthetic bacteria body weight survival rate digestive enzyme activities water quality |
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