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球孢白僵菌MZ041016菌株对桔小实蝇的毒力测定
引用本文:袁盛勇,孔 琼,马艳粉,李正跃,肖 春,普金霞.球孢白僵菌MZ041016菌株对桔小实蝇的毒力测定[J].华中农业大学学报,2010,29(2):152-156.
作者姓名:袁盛勇  孔 琼  马艳粉  李正跃  肖 春  普金霞
作者单位:1. 云南红河学院农学系,蒙自,661100
2. 云南红河学院生物系,蒙自,661100
3. 云南农业大学植物保护学院,昆明,650201
基金项目:云南省科技厅重点项目(2003NG08)资助
摘    要:以桔小实蝇成虫、幼虫和蛹为目标昆虫,利用不同分生孢子浓度的球孢白僵菌MZ041016菌株对其进行室内毒力测定。结果表明,供试菌株在室内对桔小实蝇成虫、幼虫和蛹具有较强的毒力。菌株分生孢子浓度为3.6×105个/mL时,可造成桔小实蝇各虫态大量死亡,在3.6×108个/mL孢子浓度下成虫死亡率达95.45%,致死中时间(LT50)为3.562d,第8天的致死中浓度(LC50)为2.14×105个/mL;在3.6×108个/mL孢子浓度下幼虫死亡率达95.94%,LT50为4.926d,第8天的LC50为3.03×105个/mL;3.6×108个/mL孢子浓度下蛹死亡率达78.15%,LT50为5.358d,第8天的LC50为3.31×105个/mL。

关 键 词:球孢白僵菌  桔小实蝇  毒力  
收稿时间:2009/4/10 0:00:00
修稿时间:2009/6/17 0:00:00

Detection on the Virulence of Beauveryia bassiana MZ041016 against Bactrocera dorsalis (Hendel)
YUAN Sheng-yong,KONG Qiong,MA Yan-fen,LI Zheng-yue,XIAO Chun and PU Jin-xia.Detection on the Virulence of Beauveryia bassiana MZ041016 against Bactrocera dorsalis (Hendel)[J].Journal of Huazhong Agricultural University,2010,29(2):152-156.
Authors:YUAN Sheng-yong  KONG Qiong  MA Yan-fen  LI Zheng-yue  XIAO Chun and PU Jin-xia
Institution:Department of Agronomy,College of Honghe,Yunnan Province,Mengzi 661100,China;Department of Biology,College of Honghe,Yunnan Province,Mengzi 661100,China;College of Plant Protection,Yunnan Agricultural University,Yunnan Province,Kunming 650201,China;College of Plant Protection,Yunnan Agricultural University,Yunnan Province,Kunming 650201,China;College of Plant Protection,Yunnan Agricultural University,Yunnan Province,Kunming 650201,China;Department of Agronomy,College of Honghe,Yunnan Province,Mengzi 661100,China
Abstract:The virulence of five different concentrations of isolate of Beauveryia bassiana named MZ041016 against adult, larvae and pupae of Bactrocera dorsalis (Hendal) was tested in laboratory. Large amount of Bactrocera dorsalis (Hendal) of each stage were killed at the concentration 3.6×10~5 spores/mL.The mortality of 3.6×10~5 spores/mL against adult was 95.45%, and the lethal time(LT_(50)) of this concentration was 3.562 d.The lethal concentration(LC_(50)) of eight day after treatment was 2.14×10~5 spores/mL. The mortality of 3.6×10~8 spores/mL against larvae was 95.94%, and the lethal time(LT_(50)) of this concentration was 4.926 d.The lethal concentration(LC_(50)) of eight day after treatment was 3.03×10~5 spores/mL. The mortality of 3.6×10~8 spores/mL against pupae was 78.15%, and the lethal time(LT_(50)) of this concentration was 5.358 d.The lethal concentration(LC_(50)) of eight day after treatment was 3.31×105 spores/mL. The isolate of Beauveryia bassiana named MZ041016 had virulence against adult, larvae and pupae Bactrocera dorsalis (Hendal) in laboratory, and the application prospect of it in field should be feasible. The result provided a scientific basis for the pollution-free production of fruit and biology control of Bactrocera dorsalis (Hendal) in field.
Keywords:Beauveryia bassiana  Bactrocera dorsalis (Hendel)  virulence
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