| H5N1亚型禽流感病毒新疆株NS基因的克隆和序列分析 |
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| 引用本文： | 雷程红,成进,崔尚金,李曦,沙依兰古丽,符子华,巴格得力,盛卓君,孙浩.H5N1亚型禽流感病毒新疆株NS基因的克隆和序列分析[J].中国兽医科技,2005,35(10):779-785. |
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| 作者姓名： | 雷程红 成进 崔尚金 李曦 沙依兰古丽 符子华 巴格得力 盛卓君 孙浩 |
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| 作者单位： | [1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [2]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [3]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001 [4]新疆维吾尔自治区兽医防疫总站,新疆乌鲁木齐830000 |
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| 基金项目： | 新疆维吾尔自治区攻关项目（200431101） |
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| 摘 要： | 根据已发表的H5N1禽流感病毒NS基因全序列设计了1对引物，对4株禽流感病毒新疆株的NS基因进行了RT—PCR扩增，并将其克隆到pMD18-T载体上，分别获得了全长为817、851、854、854bp的全基因序列。序列分析结果表明，新疆毒株间的核苷酸同源性为97．8％～98．9%，氨基酸同源性为96．5％～98．7％，与广东、香港和东南亚等不同地区的11个毒株比较，同源性为90．0％～99．4％；与来自野禽、猪等不同种类的11个流感毒株比较，同源性为81．4％～99．3％。系统进化树分析表明，新疆株独立分支。
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| 关 键 词： | 禽流感病毒 克隆 序列分析 H5N1 NS基因 |
| 文章编号： | 1000-6419（2005）10-0779-07 |
| 收稿时间： | 2005-04-12 |
Cloning and sequence analysis of NS gene of H5N1 avian influenza virus strains from Xinjiang |
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| LEI Cheng-hong, CHENG Jin , CUI Shang-jin , LI Xi , SHA YILAN-Guli , FU Zi-hua , Bagedeli , SHENG Zhuo-jun , SUN Hao.Cloning and sequence analysis of NS gene of H5N1 avian influenza virus strains from Xinjiang[J].Chinese Journal of Veterinary Science and Technology,2005,35(10):779-785. |
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| Authors: | LEI Cheng-hong CHENG Jin CUI Shang-jin LI Xi SHA YILAN-Guli FU Zi-hua Bagedeli SHENG Zhuo-jun SUN Hao |
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| Institution: | 1. Veterinary Research Institute, Xinjiang Academy of Animal Sciences, Uruemqi 830000, China ; 2. College of Veterinary Medicine ,Xinjiang Agricultural University, Oruemqi 830052, China; 3. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China; 4. Xinjiang General Station of Animal Epidemic Prevention , Oruemqi 830000, China |
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| Abstract: | A pair of primers specific to NS gene of four strains of H5N1 subtype influenza A virus from Xinjiang was designed on the basis of the reported gene sequence. The complimentary DNA of the NS gene was amplified by RT-PCR and cloned into pMD18-T vector. The nucleotide sequences of the NS gene of the four strains were 817,851,854 and 854 bp in length respectively. The sequence analy the nucleotide homologies among the four strains were from 97. 8% to 98. 9%, and the acids homologies among them were from 96.5% to 98.7%, the nucleotide homologies of sis showed that deduced amino the four strains with that of eleven strains from Guangdong, Hong Kong and southeastern Asia were from 90. 0% to 99.4%, and the nucleotide homologies of the four strains with that of eleven strains from wildfowls, pigs and so on were from 81.4% to 99.3%. |
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| Keywords: | influenza virus cloning sequence analysis H5N1 NS gene |
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