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| 草莓annfaf基因反义融合表达载体构建及转基因植株的获得 | |
| 引用本文： | 那杰,王关林,夏然,杨怀义.草莓annfaf基因反义融合表达载体构建及转基因植株的获得[J].中国农业科学,2006,39(3):582-586. |
| 作者姓名： | 那杰 王关林 夏然 杨怀义 |
| 作者单位： | 1. 大连理工大学生物工程学院,大连,116012;辽宁师范大学生命科学学院,大连,116029;中国科学院微生物研究所,北京,100080 2. 辽宁师范大学生命科学学院,大连,116029 3. 中国科学院微生物研究所,北京,100080 |
| 摘 要： | 【目的】建立研究草莓果实膜联蛋白基因annfaf功能体系。【方法】利用反义基因技术将从草莓成熟果实中分离克隆的annfaf基因定向克隆至植物中间表达载体pROKⅡ，构建了annfaf反义融合基因植物表达载体pRRJ4，通过根癌农杆菌EHA105将其导入草莓基因组。对获得的抗性植株进行PCR和Southern检测分析。【结果】表明该反义基因已整合到草莓基因组中。【结论】可进一步筛选annfaf基因表达缺陷型植株，用于探讨annfaf在非跃变型果实草莓成熟中的调控作用，为培育草莓新品种提供中间材料。 |
| 关 键 词： | 草莓 annfaf反义基因 植物表达载体 转基因植株 |
| 收稿时间： | 2005-10-23 |
| 修稿时间： | 2005-10-232005-12-13 |
Construction of Anti-Sense Gene of annfaf and Genetic Transformation of Strawberry |
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| NA Jie,WANG Guan-lin,XIA Ran,YANG Huai-yi.Construction of Anti-Sense Gene of annfaf and Genetic Transformation of Strawberry[J].Scientia Agricultura Sinica,2006,39(3):582-586. | |
| Authors: | NA Jie WANG Guan-lin XIA Ran YANG Huai-yi |
| Institution: | 1.Bioengineering College, Dalian University of Technology, Dalian 116012; 2. College of Life Science, Liaoning Normal University, Dalian 116029; 3. institute of Microbiology, Chinese Academy of Sciences, Beijing 100080 |
| Abstract: | Objective] In order to establish an experimental system for annfafgene function study in strawberry. Method] Utilizing anti-sense technology, annfafgene which was cloned from strawberry fruit, was inserted directionally into pROK II, a plant intermediate expression vector, to construct an anti-sense fusion gene and a plant expression vector pRRJ4. Genetic transformation to strawberry was mediated by EHA105. Transgenic assays were performed using PCR and Southern blot. Result] The anti-sense fusion annfaf gene had been integrated into strawberry genome. Conclusion] An experimental system for selecting deficient transgenic plants which might benefit for further study on the mechanism of maturation of non-climacteric fruits such as strawberry was established. Also these deficient plants can be used as intermediate materials for fresh-keeping strawberry breeding. |
| Keywords: | Strawberry (Fragaria×ananassa Duch Anti-sense gene of annfaf Plant expression vector Transgenic plants |
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