| 黑龙江野鲤与德国镜鲤杂交组合的亲子鉴定分析 |
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| 引用本文： | 葛会争,贾智英,李池陶,柏盈盈,武 耀,石连玉.黑龙江野鲤与德国镜鲤杂交组合的亲子鉴定分析[J].上海海洋大学学报,2012,21(4):489-494. |
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| 作者姓名： | 葛会争 贾智英 李池陶 柏盈盈 武 耀 石连玉 |
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| 作者单位： | 中国水产科学研究院黑龙江水产研究所;中国水产科学研究院黑龙江水产研究所;中国水产科学研究院黑龙江水产研究所;中国水产科学研究院黑龙江水产研究所;中国水产科学研究院黑龙江水产研究所;中国水产科学研究院黑龙江水产研究所 |
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| 基金项目： | 现代农业产业技术体系建设专项资金(CARS-46-02)；黑龙江省自然科学基金项目（C201044）；黑龙江水产研究所基本科研业务费专项资金（201005）；国家高技术研究发展计划（2011AA100402） |
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| 摘 要： | 利用微卫星多态性进行鱼类亲子鉴定,在鱼类选育中具有重要意义。采用8个微卫星标记对黑龙江野鲤♀×德国镜鲤♂(正交组合),及德国镜鲤♀×黑龙江野鲤♂(反交组合)进行亲子鉴定。结果显示:(1)正交组中父母本和子代观测杂合度分别为0.607 1、0.714 3和0.681 8,多态信息含量依次为0.442 1、0.281 3和0.553 5;反交组中父母本和子代观测杂合度分别为0.708 3、0.538 6和0.743 8,多态信息含量依次为0.673 5、0.375 9和0.624 1,表明8个位点具有较高的多态性。(2)正交组中,8个位点累计排除率为99.92%,亲本与子代的配对率为98.00%(置信度为95%);反交组中,8个位点累计排除率为99.99%,亲本与子代的配对率为99.00%(置信度为95%)。(3)关于使用微卫星标记数与鉴定率的探讨,对3、4、5、6、7、8个微卫星标记时的累计排除率和亲子配对率进行比较,结果表明,正反交组分别利用6和8个多态性良好的引物就能正确鉴定。研究亮点:(1)将亲子鉴定技术运用在黑龙江野鲤和德国镜鲤杂交育种过程中,能够更加准确地构建家系,有效地避免近亲交配,防止种质退化;(2)对微卫星标记数与鉴定率的探讨结果表明,正反交组分别利用6和8个多态性良好的引物就能正确鉴定。
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| 关 键 词： | 鲤 微卫星多态性 亲子鉴定 遗传结构 |
Parentage identification of Heilongjiang wild carp and German mirror carp cross combinations on microsatellite DNA markers |
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| GE Hui-zheng,JIA Zhi-ying,LI Chi-tao,BAI Ying-ying,WU Yao and SHI Lian-yu.Parentage identification of Heilongjiang wild carp and German mirror carp cross combinations on microsatellite DNA markers[J].Journal of Shanghai Ocean University,2012,21(4):489-494. |
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| Authors: | GE Hui-zheng JIA Zhi-ying LI Chi-tao BAI Ying-ying WU Yao and SHI Lian-yu |
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| Institution: | 1 (1.Heilongjiang Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,Heilongjiang,China;2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | A set of 8 microsatellite markers were used for parentage identification in obverse cross group of Heilongjiang carp♀×German mirror carp♂,and inverse cross group of German mirror carp♀×Heilongjiang carp♂.The results showed that:(1) observed heterozygosity values of parents and offspring were 0.607 1,0.714 3,0.681 8 and 0.708 3,0.538 6,0.743 8,and polymorphism information content were 0.442 1,0.281 3,0.553 5 and 0.673 5,0.375 9,0.624 1 in two groups,indicating that all 8 microsatellite loci showed polymorphism.(2) In the obverse cross group,the accumulative exclusion rate using 8 microsatellite loci was 99.92% and the appraisal ability was 98.00% while confidence was 95%.In the inverse cross group,the accumulative exclusion rate was 99.99% and the appraisal ability was 99.00%.(3) about relation between microsatellite markers and appraisal ability,we compared the accumulative exclusion rates and the appraisal ability using 3,4,5,6,7,8 microsatellite markers.6 and 8 high polymorphism markers were successfully applied in paternity determination of two cross groups. |
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| Keywords: | common carp microsatellite polymorphism paternity identification genetic structure |
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