| 刀鲚不同组织的乳酸脱氢酶同工酶及DNA含量研究 |
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| 引用本文： | 徐钢春,董晶晶,聂志娟,徐跑,顾若波.刀鲚不同组织的乳酸脱氢酶同工酶及DNA含量研究[J].上海海洋大学学报,2012,21(4):481-488. |
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| 作者姓名： | 徐钢春 董晶晶 聂志娟 徐跑 顾若波 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心 农业部淡水渔业和种质资源利用重点实验室;南京农业大学 渔业学院;中国水产科学研究院淡水渔业研究中心 农业部淡水渔业和种质资源利用重点实验室;中国水产科学研究院淡水渔业研究中心 农业部淡水渔业和种质资源利用重点实验室;中国水产科学研究院淡水渔业研究中心 农业部淡水渔业和种质资源利用重点实验室 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助项目（2011JBFA17）；国家科技支撑计划项目（2012BAD26B05）；公益性行业科研专项（201203065）；江苏省科技支撑计划（农业）项目（BE2011411） |
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| 摘 要： | 采用同工酶技术和流式细胞术研究了刀鲚肝脏、眼睛、肾脏、肌肉、鳃和血清6个组织的乳酸脱氢酶(LDH)及尾鳍、鳃、肌肉、性腺和肝脏5个组织的细胞核DNA含量,旨在为刀鲚遗传背景和种质标准的建立提供技术参数。结果显示:(1)刀鲚不同组织的LDH同工酶呈现一定的组织特异性,眼睛是LDH表达较为典型的组织;除肌肉和血清没有LDH2条带外,其余组织均出现了5条同工酶酶带,并且相对迁移率一致,各组织中均未见LDH-c基因的表达。(2)刀鲚鳃、肌肉和肝脏3个组织的细胞核DNA含量不存在显著差异(P0.05),但都显著低于尾鳍、卵巢细胞DNA含量(P0.05);测定的鳃、肌肉和肝脏组织细胞核的DNA含量与单倍体的精子细胞的比值依次为2.16、2.19和2.22,即刀鲚为二倍体鱼类。此外,肝脏细胞容易从组织上脱落,较易制成单细胞悬液,是用流式细胞仪进行倍性分析的适宜试材;如果要保持生物体健康存活状态,则剪取小部分尾鳍进行倍性分析为宜。研究亮点:首次报道了刀鲚的乳酸脱氢酶同工酶酶带相对迁移率、组织分布、活性扫描及细胞核DNA含量。研究发现,刀鲚眼睛是LDH表达较为典型的组织;刀鲚是二倍体鱼类,肝脏细胞是进行倍性分析的适宜试材,这为刀鲚遗传背景和种质标准的建立提供了科学依据。
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| 关 键 词： | 刀鲚 乳酸脱氢酶 DNA含量 |
Studies on lactate dehydrogenase isozymes and DNA content in different tissues of Coilia nasus |
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| XU Gang-chun,DONG Jing-jing,NIE Zhi-juan,XU Pao and GU Ruo-bo.Studies on lactate dehydrogenase isozymes and DNA content in different tissues of Coilia nasus[J].Journal of Shanghai Ocean University,2012,21(4):481-488. |
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| Authors: | XU Gang-chun DONG Jing-jing NIE Zhi-juan XU Pao and GU Ruo-bo |
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| Institution: | 1(1.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu,China;2.College of Fisheries,Nanjing Agriculture University,Wuxi 214081,Jiangsu,China) |
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| Abstract: | The aim of this study was to provide technical parameters for the foundation of genetic background and resources in Coilia nasus.In the present study,lactate dehydrogenase isozymes(LDH) in liver,eye,kidney,muscle,gill and serum,and DNA content in tail fin,gill,muscle,gonad and liver were studied by isozyme and flow cytometry techniques.The results indicated that:(1) The LDH in different tissues had a tissue specialty.All the tissues exhibited five isozyme bands and same relative migration rate,except for LDH-c expression.No LDH 2 bands were found in muscle and serum.(2) No significant differences were observed in DNA content in gill,muscle and liver(P > 0.05),but DNA content in those tissues was significantly lower than that in the tail fin and ovary cells(P < 0.05).The ratio of DNA content in gill,muscle and liver and haploid spermatide were 2.16,2.19 and 2.22,respectively.Moreover,the hepatocytes which were easily isolated from tissue and made into single cell suspension were good materials for ploidy analysis by flow cytometry. |
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| Keywords: | Coilia nasus lactate dehydrogenase isoenzymes DNA content |
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