| 骏枣不同株系间的枣疯病抗性多样性 |
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| 引用本文： | 肖京,杨艳荣,赵锦,刘孟军.骏枣不同株系间的枣疯病抗性多样性[J].中国农业科学,2013,46(23):4977-4984. |
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| 作者姓名： | 肖京 杨艳荣 赵锦 刘孟军 |
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| 基金项目： | 河北省自然科学基金（C2009000534）、河北省科技支撑计划（11230606D-6） |
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| 摘 要： | 【目的】揭示骏枣品种内枣疯病抗性多样性，筛选高抗枣疯病的骏枣株系。【方法】以骏枣的16个株系为试材，通过田间调查和PCR检测，研究不同株系在嫁接侵染枣疯病后的症状表现和病原侵染情况；利用“合成-合理满意度”和多维价值理论的合并原则，对不同株系果实的相对含水率、单果重、可食率、果形指数、Vc含量、可溶性糖和可滴定酸含量等性状指标进行综合评价；采用AFLP技术对不同株系进行基因组遗传多样性分析。【结果】不同株系对枣疯病抗性表现出显著差异，可分为感病型、延迟感病型、延迟抗病型和抗病型4种类型，T15、J2、J5、J8这4个骏枣株系高抗枣疯病，其中，T15、J5和J8的果实综合性状在骏枣株系中亦居于较高水平；进一步的AFLP分析发现，不同抗性类型间在基因组水平上存在一定差异。【结论】筛选出了4个抗枣疯病骏枣株系，其中3个株系果实综合品质良好。
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| 关 键 词： | 骏枣 枣疯病 症状调查 植原体 果实品质 抗性品系 |
| 收稿时间： | 2013-08-05 |
Diversity of Resistance to Jujube Witches’ Broom Disease Among the Strains of Ziziphus jujuba Mill. Junzao |
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| XIAO Jing-,YANG Yan-Rong-,ZHAO Jin-,LIU Meng-Jun-.Diversity of Resistance to Jujube Witches’ Broom Disease Among the Strains of Ziziphus jujuba Mill. Junzao[J].Scientia Agricultura Sinica,2013,46(23):4977-4984. |
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| Authors: | XIAO Jing- YANG Yan-Rong- ZHAO Jin- LIU Meng-Jun- |
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| Institution: | 1.Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, Hebei;
2.College of Life Science, Agricultural University of Hebei, Baoding 071000, Hebei |
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| Abstract: | 【Objective】Sixteen strains of Z. jujuba Junzao were collected and evaluated, aiming at revealing the diversity of resistance to JWB within cultivar, screening excellent strains with high resistance. 【Method】The JWB symptom development and phytoplasma infection were studied through field investigation and PCR analysis. The fruit economical characters, including the rate of water content, fruit weight, fruit shape index, ratio of edibility, sugar content, Vc content and titratable acidity, were comprehensively evaluated among different strains using synthesis-reasonable satisfaction and multivalue theory merging rule. The genetic diversity of Junzao strains were analyzed by AFLP. 【Result】 Different strains of Junzao showed significant differences in JWB resistance. According to the situations of disease symptom development and phytoplasma infection, the strains were divided into four groups: sensitive, delayed sensitive, delayed resistant and resistant, and 4 strains of Junzao (T15, J2, J5, J8) with very high resistance to JWB were screened out. In the resistant group, the fruit quality of T15, J5 and J8 was also at higher level among the sixteen strains. AFLP analysis showed that there had significant differences in DNA level among different strains of Junzao. 【Conclusion】 Four strains of Junzao (T15, J2, J5, J8) with very high resistance to JWB were screened out, and three of them (T15, J5 and J8) also have an excellent fruit quality. |
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| Keywords: | Junzao jujube witches&rsquo broom symptom investigation phytoplasma fruit quality resistant strains |
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