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| 首次发现我国柑橘小实蝇沃尔巴克氏体的共生 | |
| 引用本文： | 孙晓,李志红,梁广勤,陈洪俊,陈乃中.首次发现我国柑橘小实蝇沃尔巴克氏体的共生[J].植物检疫,2004,18(6):321-323. |
| 作者姓名： | 孙晓 李志红 梁广勤 陈洪俊 陈乃中 |
| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100094 2. 广州出入境检验检疫局 3. 中国检验检疫科学院动植物检疫研究所 |
| 摘 要： | 沃尔巴氏体(Wolbachia)是广泛分布于节肢动物体内的一类共生细菌,它们可以调控宿主的生殖活动.本研究利用沃尔巴克氏体wsp基因的一对通用引物(81F,691R),成功地从我国柑橘小实蝇雄成虫的总DNA中扩增到一段400bp左右的wsp基因片段,通过wsp基因的特异性扩增首次证实了沃尔巴克氏体在我国柑橘小实蝇雄虫体内的共生. |
| 关 键 词： | 沃尔巴克氏体 柑橘小实蝇 wsp基因 共生 |
Detection of wolbachia in Bactrocera (B.) dorsalis (Hendel) of China |
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| Sun Xiao,Li Zhihong,Liang Guangqin,Chen Hongjun,Chen Naizhong.Detection of wolbachia in Bactrocera (B.) dorsalis (Hendel) of China[J].Plant Quarantine,2004,18(6):321-323. | |
| Authors: | Sun Xiao Li Zhihong Liang Guangqin Chen Hongjun Chen Naizhong |
| Abstract: | Wolbachia is a common and wide spread group of symbiotic bacteria found in reproductive tissue of arthropods. These bacteria are transmitted through the cytoplasm of eggs and have evolved various mechanisms for manipulating reproduction of their hosts. In this study, PCR was used to test the presence of Wolbachia, by using general wsp primers (81F,691R). Wolbachia was detected for the first time harbouring in the male of Bactrocera (B.) dorsalis (Hendel) of China. |
| Keywords: | Wolbachia Bactrocera (B ) dorsalis (Hendel) wsp gene symbiotic bacteria |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |