| 降解亚硝酸盐的大山芽孢杆菌JY-1的分离、鉴定及其作用 |
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| 引用本文： | 周敏,宁文,陈红菊,季相山,赵燕.降解亚硝酸盐的大山芽孢杆菌JY-1的分离、鉴定及其作用[J].中国水产科学,2022,29(2):284-294. |
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| 作者姓名： | 周敏 宁文 陈红菊 季相山 赵燕 |
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| 作者单位： | 山东农业大学动物科技学院,山东 泰安 271018 |
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| 基金项目： | 山东省现代农业产业技术体系项目(SDAIT-13)； 山东省农业重大应用技术创新项目(SD2019YY008)； 山东省重点研发计划项目(2021CXGC010806). |
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| 摘 要： | 为得到稳定高效降解亚硝酸盐的益生菌,通过菌株富集分离培养、菌株鉴定、发酵培养基优化等实验对菌株进行了分离、鉴定,对菌株降亚硝酸盐效果进行了分析,并克隆了亚硝酸盐还原酶基因.结果显示,从养殖池塘、污水处理厂等5处混合水体中分离、筛选出一株具有亚硝酸盐降解能力的菌株,命名为JY-1.菌株生理生化和16S rDNA序列分析显...
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| 关 键 词： | 大山芽孢杆菌 亚硝酸盐 养殖水体 分离 鉴定 |
Isolation, identification and function of a nitrite-degrading strain JY-1 |
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| ZHOU Min,NING Wen,CHEN Hongju,JI Xiangshan,ZHAO Yan.Isolation, identification and function of a nitrite-degrading strain JY-1[J].Journal of Fishery Sciences of China,2022,29(2):284-294. |
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| Authors: | ZHOU Min NING Wen CHEN Hongju JI Xiangshan ZHAO Yan |
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| Institution: | College of Animal Science and Technology,Shandong Agricultural University,Tai?an 271018 ,China |
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| Abstract: | In the present study, to isolate and screen a probiotic that degrades nitrite stably and efficiently, we carried out experiments on enrichment, isolation, culture, and identification of a suitable strain. Then, biological characteristics of the identified strain were determined through optimization of fermentation medium, analysis of nitrite degrading effect, and gene cloning of nitrite reductase. The strain was isolated and screened from five mixed water bodies, including aquaculture projects and wastewater treatment plants, and named JY-1. Additionally, the strain JY-1 was identified using 16S rDNA gene sequence analysis and physiological and biochemical characteristics. The results revealed strain JY-1 to be Bacillus gaemokensis. Optimization of fermentation conditions for sporulation of JY-1 strain indicated that the best carbon source for JY-1 was glucose and the best nitrogen source was tryptone. Analysis of the nitrite-degrading effect of JY-1 showed that JY-1 could degrade 0.6 mg/L nitrite in aquaculture water to 0.0 mg/L in 3 h. Moreover, the safety evaluation test suggested that no hemolysis was found, and the high concentration of JY-1 (109 CFU/mL) had no significant effect on the survival of Nile tilapia. Based on the results of experiments of gene cloning of nitrite reductase and the effect of JY-1 on ammonia, nitrite, and total nitrogen in water bodies, it is preliminarily speculated that enzymatic degradation, operated by JY-1, was the pathway through which nitrite was degraded and removed from water. In summary, this study provides an important basis for the preparation of microecological preparations with high efficiency in degrading nitrite. |
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