| 金线莲增殖和生根培养条件的优化 |
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| 引用本文： | 梁丽凤,林晓林,伍宇雁,莫艳坤,周玲艳.金线莲增殖和生根培养条件的优化[J].仲恺农业工程学院学报,2014(3):5-8,13. |
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| 作者姓名： | 梁丽凤 林晓林 伍宇雁 莫艳坤 周玲艳 |
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| 作者单位： | 仲恺农业工程学院生命科学学院 |
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| 摘 要： | 为了优化金线莲(Anoectochilus formosanus Hayata)增殖和生根培养条件,将金线莲无菌苗的顶芽和带节茎段接种到不同种类和浓度的激素以及添加物组合的培养基中进行增殖和生根培养,以筛选出最适合金线莲增殖和生根的培养基.结果表明,金线莲最佳增殖培养基为MS+6-BA 3.0 mg/L+NAA 0.6 mg/L+玉米素(Zeatin,ZT)0.8 mg/L+香蕉泥100 g/L,顶芽和腋芽的增值倍数分别为3.82和3.13;生根培养基以MS+NAA0.5 mg/L+IBA 0.5 mg/L最好,生根率达80%.
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| 关 键 词： | 金线莲(Anoectochilus formosanus Hayata) 增殖 生根 |
Optimization of proliferation and rooting culture of Anoectochilus formosanus |
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| LIANG Lifeng;LIN Xiaolin;WU Yuyan;MO Yankun;ZHOU Lingyan.Optimization of proliferation and rooting culture of Anoectochilus formosanus[J].Journal of Zhongkai University of Agriculture and Technology,2014(3):5-8,13. |
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| Authors: | LIANG Lifeng;LIN Xiaolin;WU Yuyan;MO Yankun;ZHOU Lingyan |
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| Institution: | LIANG Lifeng;LIN Xiaolin;WU Yuyan;MO Yankun;ZHOU Lingyan;College of Life Sciences,Zhongkai University of Agriculture and Engineering; |
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| Abstract: | The aseptic plantlets of Anoectochilus formosanus were used as material in this paper .The opti-mum media for proliferation and rooting were screened while the terminal buds and stem with axillary of A.formosanus were inoculated onto medium with different kind and concentration of hormones and addi -tives.The results showed that the medium of MS ＋6-BA 3 mg/L ＋NAA 0.6 mg/L ＋Zeatin （ ZT） 0.8 mg/L＋banana 100 g/L was most suitable for proliferation of A.formosanus, and that the rate of proliferation for terminal buds and axillary buds was 3.85 and 3.07 respectively .The medium of MS＋NAA 0.5 mg/L＋IBA 0.5 mg/L was most suitable for rooting of A.formosanus, and the rate of rooting reached 80%. |
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| Keywords: | Anoectochilus formosanus proliferation rooting |
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