| 亚洲璃眼蜱唾液腺多肽Ha-11的鉴定、表达和生物学功能的初步研究 |
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| 引用本文： | 张冰炳,龚海燕,周勇志,曹杰,张厚双,周金林.亚洲璃眼蜱唾液腺多肽Ha-11的鉴定、表达和生物学功能的初步研究[J].中国动物传染病学报,2014(3):48-52. |
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| 作者姓名： | 张冰炳 龚海燕 周勇志 曹杰 张厚双 周金林 |
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| 作者单位： | 上海师范大学生命与环境科学学院;中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室 |
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| 基金项目： | 卫生行业科研专项经费资助项目(201202019) |
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| 摘 要： | 本研究对亚洲璃眼蜱多肽Ha-11的编码基因进行了克隆、表达和初步的功能分析。该基因开放阅读框（open reading frame,ORF）为372 bp,编码123个氨基酸,其中含23个氨基酸的信号肽。将去除信号肽的编码区基因亚克隆至pGEX-4T-1载体,大肠杆菌中诱导表达,获得了37 kDa重组蛋白。抗重组Ha-11蛋白的免疫血清识别半饱血雌蜱的唾液腺中约11 kDa的天然蛋白。Real-time PCR结果表明该基因在亚洲璃眼蜱的各发育阶段、各组织中均有表达,但以若蜱表达量最高,而组织中以淋巴液表达量最高。重组蛋白在体外可以抑制脾细胞增殖和细胞因子IL-2、IFN-γ的分泌。研究结果显示Ha-11在蜱吸血过程中可能具有抗炎作用。
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| 关 键 词： | 唾液腺 亚洲璃眼蜱 多肽Ha-11 鉴定 |
EXPRESSION AND PRIMARY CHARACTERIZATION OF HA-11 POLYPEPTIDE FROM HYALOMMAASIATICUM |
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| ZHANG Bing-bing;GONG Hai-yan;ZHOU Yong-zhi;CAO Jie;ZHANG Hou-shuang;ZHOU Jin-lin.EXPRESSION AND PRIMARY CHARACTERIZATION OF HA-11 POLYPEPTIDE FROM HYALOMMAASIATICUM[J].Chinese Journal of Animal Infectious Diseases,2014(3):48-52. |
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| Authors: | ZHANG Bing-bing;GONG Hai-yan;ZHOU Yong-zhi;CAO Jie;ZHANG Hou-shuang;ZHOU Jin-lin |
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| Institution: | ZHANG Bing-bing;GONG Hai-yan;ZHOU Yong-zhi;CAO Jie;ZHANG Hou-shuang;ZHOU Jin-lin;College of Life and Environment Science, Shanghai Normal University;Key Laboratory of Animal Parasitology of the Ministry of Agriculture,Shanghai Veterinary Research Institute, CAAS; |
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| Abstract: | In the present study, we reported cloning, expression and primary biological identification of Ha-11gene from Hyalomma asiaticum. The ORF of Ha-11gene was 372 bp encoding 123 amino acid residues including a signal peptide of 23 amino acid residues. A 37 kDa recombinant protein was obtained from E.coli DH5αusing pGEX-4T-1 as expression. A specific band about 11kDa was detected in the salivary gland of female ticks in Western blot using anti-recombinant Ha-11serum. The result of Real-time PCR indicated that Ha-11 was expressed in all of developmental stages and tissues of adult female ticks with the highest expression in nymphs and hemolymphs. Recombinant Ha-11 protein significantly suppressed the proliferation of spleen cells and secretion of cytokines including IFN-γand IL-2 invivo. The results obtained here suggested that recombinant protein might be involved in suppression of inflammation. |
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| Keywords: | Salivary gland Hyalommaasiaticum Ha-11 polypeptide identification |
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