| 蝴蝶兰外植体酚类物质和活性氧代谢与组培褐变的关系 |
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| 引用本文： | 赵滢,杨树华,葛维亚,李秋香,陈红霞,葛红.蝴蝶兰外植体酚类物质和活性氧代谢与组培褐变的关系[J].园艺学报,2010,37(6):963-970. |
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| 作者姓名： | 赵滢 杨树华 葛维亚 李秋香 陈红霞 葛红 |
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| 作者单位： | （1中国农业科学院蔬菜花卉研究所，北京100081；2西南大学园艺园林学院，重庆400716） |
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| 基金项目： | 国家'863'项目，国家'十五'科技攻关项目，农业部园艺作物遗传改良重点开放实验室项目 |
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| 摘 要： | （1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China）
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| 关 键 词： | 蝴蝶兰 组织培养 褐变 酚类 活性氧 |
| 收稿时间： | 2009-12-02 |
The Metabolism of Phenolics and Reactive Oxygen Species in Relation to the Explant Browning Differences Among the Varieties of Phalaenopsis During the Tissue Culture |
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| ZHAO Ying,YANG Shu-hua,GE Wei-ya,LI Qiu-xiang,CHEN Hong-xia,GE Hong.The Metabolism of Phenolics and Reactive Oxygen Species in Relation to the Explant Browning Differences Among the Varieties of Phalaenopsis During the Tissue Culture[J].Acta Horticulturae Sinica,2010,37(6):963-970. |
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| Authors: | ZHAO Ying YANG Shu-hua GE Wei-ya LI Qiu-xiang CHEN Hong-xia GE Hong |
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| Abstract: | The parameters of phenolic and reactive oxygen metabolism were measured in three varieties of Phalaenopsis，i.e. B3，15 and F5 to study the physiological mechanisms of explant browning as well as genotype differences. The total phenol contents of the three varieties were positively correlated with their PAL and POD activities. B3 with the lowest phenol content showed the highest browning degree，PPO activity and MDA content. F5 with highest phenol content had the lowest browning degree，as well as the lowest PPO activity and MDA content. Moreover，SOD and APX activities in F5 were the highest among the three varieties. These results suggested that the browning degree was not directly related to the total phenol content and that explant browning may be due to the phenol oxidation which catalyzed by PPO. Meanwhile，explant browning reflected the membrane damage caused by lipid peroxidationduring the tissue culture of Phalaenopsis. The differences in the activities of PPO，SOD and APX possibly might be the main aspects that caused the genotype differences in tissue browning. |
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| Keywords: | Phalaenopsis tissue culture browning phenolics reactive oxygen species |
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