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| 猪SLA-DQA基因外显子4多态性分析 | |
| 引用本文： | 孔晶晶,滚双宝,杨巧丽,王国旺,刘丽霞,李峰松,姜天团,封洋,赵生国.猪SLA-DQA基因外显子4多态性分析[J].华北农学报,2012,27(3):86-90. |
| 作者姓名： | 孔晶晶 滚双宝 杨巧丽 王国旺 刘丽霞 李峰松 姜天团 封洋 赵生国 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃兰州,730070 2. 甘肃农业大学动物科学技术学院,甘肃兰州730070;甘肃省现代养猪工程技术研究中心,甘肃兰州730070 3. 中国人民解放军63600部队后勤部,甘肃酒泉,732750 |
| 基金项目： | 甘肃省农牧厅生物技术专项，DQA和DRA基因与仔猪腹泻死亡的分子标记技术，高繁殖力猪新品系选育研究，甘肃省优质猪肉生产配套技术研究与示范项目 |
| 摘 要： | 采用PCR-SSCP及克隆测序技术对大白、长白、杜洛克共210头仔猪SLA-DQA基因第4外显子进行多态性检测,以探讨猪SLA-DQA基因的遗传特性。结果显示:共获得4种等位基因(A、B、C、D),6种基因型(AA、AB、CC、CD、BB、BC)。6种基因型在杜洛克猪中都被检测到,而在大白猪中只检测到AA、AB、CC 3种基因型,在长白猪中未检测到BC基因型。A等位基因和AA基因型的频率在3个群体中最高,为优势基因和优势基因型。对不同SSCP带型的对应片段进行测序分析,共发现3个核苷酸突变位点(5 126 bp A→G,5 158 bp A→T,5 233 bp G→A),其中,5126 bp处A→G导致氨基酸由异亮氨酸变为缬氨酸。经χ2适合性检验,3个猪品种在此位点上都偏离了Hardy-Wein-berg平衡状态(P<0.05)。群体遗传学分析发现:长白猪、大白猪多态信息含量(PIC)分别为0.3233,0.4548,属于中度多态(0.25 |
| 关 键 词： | DQA基因 多态性 PCR-SSCP 等位基因 |
Analyzing Polymorphism of SLA-DQA Gene exon4 |
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| KONG Jing-jing , GUN Shuang-bao , YANG Qiao-li , WANG Guo-wang , LIU Li-xia , LI Feng-song , JIANG Tian-tuan , FENG Yang , ZHAO Sheng-guo.Analyzing Polymorphism of SLA-DQA Gene exon4[J].Acta Agriculturae Boreali-Sinica,2012,27(3):86-90. | |
| Authors: | KONG Jing-jing GUN Shuang-bao YANG Qiao-li WANG Guo-wang LIU Li-xia LI Feng-song JIANG Tian-tuan FENG Yang ZHAO Sheng-guo |
| Institution: | 1,2(1.Faculty of Animal Sci-tech Gansu Agricultural University,Lanzhou 730070,China; 2.Modern Pigs Engineering Technology Research Center of Gansu,Lanzhou 730070,China; 3.General Logistics Department of the Chinese People′s Liberation Army of 63600 Troops,Jiuquan 732750,China) |
| Abstract: | The polymorphisms of the fourth exon of SLA-DQA gene were detected by PCR-SSCP,aiming to get molecular genetics information about SLA-DQA gene.The results showed that there were 4 alleles and 6 genotypes in Landrace,Large white and Duroc breeds(210 individuals).6 genotypes(A,AB,CC,CD,BB,BC)was detected in Duroc,AA,AB,CC in Large white,AA,AB,CC,CD,BB in Landrace.alleles A was the predominant allele,AA was the predominant genotype in three breeds.There are 3 mutations in exon 4,(A→G) at 5126bp,(A→T)at 5158bp and(G→A)at 5233bp respectively.(A→G)mutation leads to the changes of encoding amino acid,I→V on protein level.This polymorphic locus in three breeds is deviated from Hardy-Weinberg equilibrium by χ2 test(P <0.05).The polymorphism information content of Landrace and Large white are 0.3233 and 0.4548.respectively indicates moderate polymorphism(0.25 |
| Keywords: | DQA gene P olymorphism PCR-SSCP Allele |
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