| 中草药及其配伍对嗜水气单胞菌的抑菌作用 |
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| 作者姓名： | 张文青 龚一富 金思 孙喆君 章丽 童丽娟 |
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| 作者单位： | 宁波大学生命科学与生物工程学院应用海洋生物技术教育部重点实验室,315211 |
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| 基金项目： | 宁波市农业科技攻关项目，宁波大学学科项目 |
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| 摘 要： | 采用二倍稀释法测定了86种中草药对嗜水气单胞菌Aeromonas hydrophila的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),并采用正交试验法研究了对嗜水气单胞菌具有较强抑制作用的中草药配伍。结果表明,五倍子、五味子和乌梅对嗜水气单胞菌的抑制作用最强,最小抑菌浓度和最小杀菌浓度均为12.5mg/ml,可作为防治水产养殖动物嗜水气单胞菌病害的首选中草药。正交试验结果表明,儿茶、五倍子、五味子和乌梅4种药分别以4∶2∶4∶1和1∶2∶2∶4的比例配伍时对嗜水气单胞菌具有最佳抑菌和杀菌作用。
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| 关 键 词： | 水产养殖 中草药 嗜水气单胞菌 最小抑菌浓度 最小杀菌浓度 正交试验 |
| 收稿时间： | 2010/2/9 0:00:00 |
| 修稿时间： | 2010/5/25 0:00:00 |
Antimicrobial tests of Chinese herbal medicines and their mixtures on Aeromonas hydrophila |
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| Authors: | ZHANG Wen-qing GONG Yi-fu JIN Si SUN Zhe-jun ZHANG Li TONG Li-juan |
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| Institution: | ZHANG Wen-qing GONG Yi-fu* JIN Si SUN Zhe-junZHANG Li TONG Li-juan (Key Laboratory of Applied Marine Biotechnology, Ministry of Education; College of Life Science and Biotechnology, Ningbo University, 315211) |
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| Abstract: | The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of 86 kinds of Chinese herbal medicines (CHMs) on Aeromonas hydrophila were examined in vitro by the microdilution method. Then the optimum mixtures of CHMs which can inhibit the A. hydrophila were found through orthogonal test design. The result showed that Galla chinensis, Fructus schisandrae and Fructus mume had the strongest inhibitory effect on A. hydrophila,with the MIC and MBC as 12.5 mg/ml. In conclusion, G. chinensis, F. schisandrae and F. mume were the better drugs that can cure the disease caused by the A. hydrophila in aquatic animals. The results of orthogonal tests showed that the mixture of Acacia catechu, G. chinensis, F. schisandrae and F. mume with the proportion of 4∶2∶4∶1 and 1∶2∶2∶4 had the best MIC and MBC on A. hydrophila. |
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| Keywords: | Aquaculture Chinese herbal medicine (CHM) Aeromonas hydrophilaMinimal inhibitory concentration (MIC) Minimal bactericidal concentration (MBC) Orthogonal test design |
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