| 来源和质量不同对山羊卵母细胞体外成熟率的影响 |
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| 引用本文： | 刘海军,马群,王儒,郑梓,张文昌.来源和质量不同对山羊卵母细胞体外成熟率的影响[J].华北农学报,2012,27(2):117-120. |
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| 作者姓名： | 刘海军 马群 王儒 郑梓 张文昌 |
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| 作者单位： | 1. 天津市畜牧兽医研究所,天津,300112
2. 福建农林大学动物科学学院,福建福州,350002 |
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| 基金项目： | 天津市科技支撑计划重大项目 |
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| 摘 要： | 比较了卵母细胞级别对体外成熟率的影响,以及促卵泡素(FSH)处理山羊对卵母细胞采集的数量和质量的影响。结果表明:卵母细胞级别对体外成熟率有显著影响,不论是来源于FSH处理卵巢还是屠宰场卵巢,均是A、B级卵母细胞的成熟率高于C级卵母细胞,差异极显著(P<0.01)。但是A、B级卵母细胞之间差异不显著;卵巢来源对卵母细胞采集的数量和质量有显著影响,A、B级卵母细胞在获卵总数中所占的比例,平均每个卵巢获卵母细胞数及A、B级卵母细胞数,均是FSH处理卵巢高于屠宰场卵巢,差异显著(P<0.05);卵巢来源对卵母细胞体外成熟率有显著影响,来自于FSH处理卵巢的卵母细胞体外成熟率高于屠宰场卵巢(81.6%比67.6%),差异极显著(P<0.01)。
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| 关 键 词： | 卵母细胞 分级 体外成熟 FSH处理 山羊 |
Effect of Source and Quality of Goat Oocytes on in vitro Maturation Rate |
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| LIU Hai-jun
,
MA Qun
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WANG Ru
,
ZHENG Zi
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ZHANG Wen-chang.Effect of Source and Quality of Goat Oocytes on in vitro Maturation Rate[J].Acta Agriculturae Boreali-Sinica,2012,27(2):117-120. |
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| Authors: | LIU Hai-jun MA Qun WANG Ru ZHENG Zi ZHANG Wen-chang |
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| Institution: | 1.Tianjin Institute of Animal Science and Veterinary Medicine,Tianjin 300112,China; 2.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
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| Abstract: | The aim of the present study was to compare the effects of oocytes grade on the rate of in vitro maturation,and the effect of the FSH-primed follicles on the numbers and quality of collecting oocytes.The result showed that:Oocytes grade had significant effects on the rate of in vitro maturation,grade A and B oocytes showed significantly higher percentage of maturation than that of Grade C,whatever the oocytes from the FSH-primed goats or from slaughthouse ovaries(P<0.01).There was no signficent difference between Grade A and B oocytes.Ovaries source had significant effects on the numbers and quality of collecting oocytes,FSH-primed ovaries was higher than slaughthouse ovaries,whatever the the percentage of Grade A and B oocytes in the total ones(P<0.05),the numbers of total oocytes(P<0.05) and Grade A and B oocytes(P<0.05) from each ovary.Ovaries source had significant effects on percentage of maturation,oocytes recovered from the FSH-primed goats showed significantly higher percentage of maturation than that of oocytes from slaughthouse ovaries(P<0.01). |
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| Keywords: | Oocytes Classification In vitro maturation FSH-primed Goats |
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