| 绵羊MSTN基因启动子区SNPs分析 |
| |
| 引用本文： | 贺三刚,陈芯,刘晨曦,刘国松,刘明军.绵羊MSTN基因启动子区SNPs分析[J].华北农学报,2012,27(3):81-85. |
| |
| 作者姓名： | 贺三刚 陈芯 刘晨曦 刘国松 刘明军 |
| |
| 作者单位： | 1. 新疆畜牧科学院 生物技术研究中心,农业部草食家畜繁育生物技术重点开放实验室,新疆维吾尔自治区动物生物技术重点实验室,新疆乌鲁木齐830000
2. 新疆畜牧科学院 生物技术研究中心,农业部草食家畜繁育生物技术重点开放实验室,新疆维吾尔自治区动物生物技术重点实验室,新疆乌鲁木齐830000;新疆农业大学农学院,新疆乌鲁木齐830052 |
| |
| 基金项目： | 新疆维吾尔自治区国际科技合作项目 |
| |
| 摘 要： | 采用测序和PCR-RFLP的方法,分析了MSTN基因启动子区在7个品种绵羊中的单核苷酸多态性,结果表明:存在2个SNP位点(-959T/C,-784G/A),表现为6种基因型(TT、TC、CC、GG、GA、AA),在-959T/C位点中,国外肉用绵羊品种陶赛特、德国肉用美利奴及我区的巴士拜羊,TT为优势基因型,地方品种绵羊巴音布鲁克羊、多浪羊和阿勒泰羊在该位点中CC为优势基因型,经χ2检验,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,这6个绵羊品种中群体的多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,特克赛尔羊在该位点未发生突变。在-784G/A位点中,除陶赛特与特克赛尔羊在该位点未发生突变,其他5个品种绵羊优势等位基因均为G等位基因。经χ2检验后,其群体的基因频率和基因型频率均处于Hardy-Weinberg平衡状态,群体遗传多态性分析表明,多浪羊、巴士拜羊和巴音布鲁克羊的群体多态信息含量(PIC)均处于0.25～0.50之间,为中度多态,而阿勒泰羊和德国肉用美利奴的群体多态信息含量(PIC)小于0.25,为低度多态。
|
| 关 键 词： | 绵羊 MSTN 启动子 PCR-RFLP 多态性 |
Analysis of SNPs in Promoter Region of Myostatin in Sheep |
| |
| HE San-gang
,
CHEN Xin
,
LIU Chen-xi
,
LIU Guo-song
,
LIU Ming-jun.Analysis of SNPs in Promoter Region of Myostatin in Sheep[J].Acta Agriculturae Boreali-Sinica,2012,27(3):81-85. |
| |
| Authors: | HE San-gang CHEN Xin LIU Chen-xi LIU Guo-song LIU Ming-jun |
| |
| Institution: | 1(1.The Key Lab of Livestock Reproduction & Breed Biotechnology of MOA,The Key Lab of Animal Biotechnology of Xinjiang,Xinjiang Academy of Biotechnological Center,Urumqi 830000,China; 2.Xinjiang Agricultural University of Agronomy College,Urumqi 830052,China) |
| |
| Abstract: | Sequencing along with PCR-RFLP was applied to analyze the polymorphism of MSTN gene promoter in seven sheep breeds.Two SNPs at-959(T/C)and-784(G/A) including six genotype(TT,TC,CC,GG,GA,AA)respectively were detected in the promoter region,At-959(T/C),the predominant genotype of TT was identified in Dorset,German Merino and Bashibai sheep,the CC in Bayinbuluke,Duolang and Aletai sheep.A chi-square analysis suggested that the allele frequencies and genotype frequencies of two polymorphisms were in Hardy-Weinberg equilibrium.Statistical analysis also revealed the variation of intermediate polymorphism information content(PIC) in six breeds(0.25
|
| |
| Keywords: | Sheep MSTN Promoter PCR-RFLP Polymorphsim |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
