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马铃薯品种大西洋试管苗愈伤诱导研究
引用本文:贺学勤,蒙美莲,李巧玲.马铃薯品种大西洋试管苗愈伤诱导研究[J].北方园艺,2009(2).
作者姓名:贺学勤  蒙美莲  李巧玲
作者单位:内蒙古农业大学,农学院,内蒙古,呼和浩特,010019
基金项目:内蒙古农业大学博士科研启动基金,内蒙古自治区高等学校科学研究项目 
摘    要:为筛选适合加工型马铃薯大西洋试管苗诱导愈伤的器官来源和培养基,以大西洋试管苗的叶和茎段为材料,分别在3种培养基上进行愈伤诱导,比较接种后10、20和40 d的出愈率及愈伤组织外观.结果表明:在接种10 d和20 d时大西洋茎段诱导愈伤的速度和出愈率均高于叶片的,在接种40 d时3种培养基均可诱导出大量的愈伤,但以MS添加3 mg/L 2,4-D和0.2mg/L KT的培养基上诱导产生的愈伤速度快、颜色亮黄、外观疏松且在随后的继代培养中不随培养时间延长而产生褐化现象.

关 键 词:马铃薯  愈伤组织  诱导

Study on Inducing Calluses of Potato cv.Atlantic in Vitro
HE Xue-qin,MENG Mei-lian,LI Qiao-ling.Study on Inducing Calluses of Potato cv.Atlantic in Vitro[J].Northern Horticulture,2009(2).
Authors:HE Xue-qin  MENG Mei-lian  LI Qiao-ling
Institution:Agronomy Faculty;Inner Mongolia Agricultural University;Huhhot;Inner Mongolia 010019;China
Abstract:In order to select suitable callus medium of potato cv.Atlantic in vitro and compare to the effect of calluses derived from different organs,attempts were made to induce calluses derived from stem segments and leaves on three different media,respectively.The results showed that stem segments of Atlantic took less time to produce calluses and the frequency of stem segment-derived calluses was higher than that of leaf-derived calluses after 10 and 20 days.The calluses derived from stem segments and leaves of ...
Keywords:Potato  Callus  Induce  
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