| 马铃薯茎段愈伤组织培养体系的优化 |
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| 引用本文： | 罗 源,陈耀锋,李春莲.马铃薯茎段愈伤组织培养体系的优化[J].西北农林科技大学学报(社会科学版),2007,35(10):159-162. |
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| 作者姓名： | 罗 源 陈耀锋 李春莲 |
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| 作者单位： | 西北农林科技大学,农学院,陕西,杨陵,712100 |
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| 基金项目： | 中德农业部科技合作项目(06-37);国家“十五”科技攻关项目（2004BA516A09） |
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| 摘 要： | 以克新12号、克新13号、东农303和早大白4种马铃薯试管苗茎段为材料,研究各品种的组织培养体系。结果发现,克新13号和克新12号的最适愈伤组织诱导培养基为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+1 mg/L 2,4-D;东农303为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+0.5 mg/L 2,4-D;早大白为MS+30 g/L蔗糖+8 g/L琼脂+2 mg/L 6-BA+0.5 mg/L 2,4-D。用上述最适诱导培养基培养,各品种的愈伤诱导率分别为100%,100%,100%和90%。克新13号愈伤组织分化的最佳培养基为MS+30 g/L蔗糖+8 g/L琼脂+4.0mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L GA3;克新12号和早大白为MS+30 g/L蔗糖+8 g/L琼脂+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA3;东农303为MS+30 g/L蔗糖+8 g/L琼脂+2.0 mg/L 6-BA+0.1 mg/LNAA+1.5 mg/L GA3。用上述最适诱导培养基培养,各品种的分化率分别为80%,90%,100%,76.7%。
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| 关 键 词： | 马铃薯 克新12号 克新13号 东农303 早大白 愈伤诱导 愈伤分化 |
| 文章编号： | 1671-9387（2007）09-0159-04 |
| 收稿时间： | 2006/8/13 0:00:00 |
| 修稿时间： | 2006-08-13 |
Optimizing for callus tissue culture system for the stem of Solanum tuberosum |
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| LUO Yuan,CHEN Yao-feng,LI Chun-lian,YU Ling,REN Hui-li,LI Ying-mei.Optimizing for callus tissue culture system for the stem of Solanum tuberosum[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(10):159-162. |
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| Authors: | LUO Yuan CHEN Yao-feng LI Chun-lian YU Ling REN Hui-li LI Ying-mei |
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| Institution: | (College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | Four potato cultivars,Kexin No.12,Kexin No.13,Dongnong303 and Zaodabai were cultivated in vitro from stem explants.The results showed that the optimal medium of callus for Kexin No.12 and Kexin No.13 was MS 30 g/L sucrose 8g/L agar 2 mg/L6-BA 1 mg/L 2,4-D;Dongnong 303 was MS 2 mg/L 6-BA 0.5 mg/L 2,4-D;the optimal medium of Zaodabai was MS 30 g/L sucrose 8 g/L agar 2 mg/L 6-BA 0.5 mg/L 2,4-D;the adventitious callus induction rate were 100%,100%,100% and 90%,respectively by using these optimal induced medium.The best medium for adventitious bud differentiation from stem callus was MS 30 g/L sucrose 8 g/L agar 4.0 mg/L 6-BA 0.1 mg/L NAA 1.0 mg/L GA3 for Kexin13;MS 30 g/L sucrose 8 g/L agar 2.0 mg/L 6-BA 0.5 mg/L NAA 1.0 mg/L GA3 for Kexin12;MS 30 g/L sucrose 8 g/L agar 2.0 mg/L 6-BA 0.1 mg/L NAA 1.5 mg/L GA3 for Dongnong 303;MS 30 g/L sucrose 8 g/L agar 2.0 mg/L 6-BA 0.5 mg/L NAA 1.0 mg/L GA3 for Zaodabai.Under these optimal induced medium,the rates of adventitious bud differentiation were 80%,90%,100%,and 76.7%,respectively. |
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| Keywords: | potato Kexin No 12 Kexin No 13 Dongnong303 Zaodabai callus induction callus differentiation |
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