| 香荚兰根腐病原菌酯酶同工酶分析* |
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| 引用本文： | 李 霞,陈丽珊,王云月.香荚兰根腐病原菌酯酶同工酶分析*[J].云南农业大学学报,2005,20(1):27-30. |
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| 作者姓名： | 李 霞 陈丽珊 王云月 |
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| 作者单位： | 云南农业大学云南省植物病理重点实验室，云南农业大学云南省植物病理重点实验室，云南农业大学云南省植物病理重点实验室 云南昆明650201，云南昆明650201，云南昆明650201 |
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| 基金项目： | 云南省科技攻关项目(2000A3-01) |
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| 摘 要： | 香荚兰根腐病是影响香荚兰生产的一个制约因素，其病原菌为尖孢镰刀菌和茄类镰刀菌。试验应用同工酶电泳技术对引起香荚兰根腐病致病性不同的12个菌株(其中尖镰孢9株，茄镰孢3株)进行酯酶同工酶谱分析。结果发现酯酶同工酶的活性区主要集中在中间带区和快带区。对12个菌株的酯酶同工酶带的Rf值进行UPGMA聚类分析，发现在相似性0.71的水平上，能将尖孢镰刀菌和茄类镰刀菌区分；在相似性0.86的水平上，能将用传统的致病性鉴定方法鉴定出的强、中、弱致病菌株及非致病菌株分开。试验的目的在于寻找与致病性/非致病性相关的同工酶标记。
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| 关 键 词： | 香荚兰 尖镰孢 茄镰孢 酯酶同工酶 |
| 文章编号： | 1004-390X(2005)01-0027-04 |
| 收稿时间： | 2004-11-9 |
Isozyme Analysis of the Causal Reagents of Vanilla Root-rot |
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| LI Xia,CHEN Li-shan,WANG Yun-yue Kunming ,China.Isozyme Analysis of the Causal Reagents of Vanilla Root-rot[J].Journal of Yunnan Agricultural University,2005,20(1):27-30. |
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| Authors: | LI Xia CHEN Li-shan WANG Yun-yue Kunming China |
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| Institution: | LI Xia,CHEN Li-shan,WANG Yun-yue Kunming 650201,China ) |
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| Abstract: | Vanilla root rot has been a limiting factor to vanilla production in most regions in which it is grown. The causal reagents of vanilla root rot were Fusarium oxysporum f.sp. vanillae and Fusarium solani. The technique of isozyme electrophoresis was applied to analyze 12 of causal reagents of vanilla root rot, including 9 isolates of F.oxysporum and 3 isolates of F.solani. The aims of this research was to find the marker related with pathotypes. The EST (esterase) electrophoresis analysis showed: EST was almost distributed in middle and fast areas. All the EST bands were clustered by Unweighted Pair Group with Mathematic Average (UPGMA). The dendrogram of UPGMA showed that F.oxysporum f.sp.vanillae and F.solani can be differentiated based on 0.71 analogical level; different pathotypes can be differentiated based on 0.86 analogical level. |
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| Keywords: | Vanilla root rot Fusarium oxysporum Fusarium solani Esterase |
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