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南宁市郊奶牛子宫内膜炎病原菌分离鉴定与耐药性分析
引用本文:凌丁,张业怀,莫文湛,王彤.南宁市郊奶牛子宫内膜炎病原菌分离鉴定与耐药性分析[J].广东农业科学,2013,40(5):111-115.
作者姓名:凌丁  张业怀  莫文湛  王彤
作者单位:广西农业职业技术学院,广西南宁,530007
基金项目:2012年度广西高等学校科研项目
摘    要:为探讨奶牛子宫内膜炎的发病因素以及为实际生产提供适合的预防治疗措施,对南宁市周边地区22头患子宫内膜炎奶牛采集子宫分泌物,对采集的样本进行病原菌的分离鉴定和药物敏感性试验.结果从22份样品中分离出葡萄球菌、大肠杆菌、链球菌、蜡样芽孢杆菌和酵母样真菌(白色念珠菌)等5类细菌共30个菌株;葡萄球菌、大肠杆菌、链球菌、蜡样芽孢杆菌对替加环素表现出高度敏感,而对红霉素、万古霉素、苯唑西林等11种抗菌药物表现出不同程度的耐药性;酵母样真菌(白色念珠菌)对两性霉素B、伊曲康唑等5种抗真菌药物高度敏感.

关 键 词:奶牛  子宫内膜炎  病原菌  分离鉴定  药敏试验  耐药性

An analysis of isolating identification and test of drug sensitivity to pathogens of cow endometritis in Nanning
LING Ding , ZHANG Ye-huai , MO Wen-zhan , WANG Tong.An analysis of isolating identification and test of drug sensitivity to pathogens of cow endometritis in Nanning[J].Guangdong Agricultural Sciences,2013,40(5):111-115.
Authors:LING Ding  ZHANG Ye-huai  MO Wen-zhan  WANG Tong
Abstract:This work was aimed to investigate etiological factors of cow endometrits and its preventions and treatments. 22 samples of secretion were collected from uteruses of cows with endometris in cow farms around Nanning, Guangxi. They were dealt with isolation and identification of pathogenic bacteria and drug sensitivity testing. The results showed that 30 bacterial strains were isolated from the 22 samples, which belonged to 5 kinds including staphylococcus, E. coli, streptococcus, Bacillus cereus and yeast-like fungi. The isolated staphylococcus, E. coli, streptococcus and Bacillus cereus strains had a high sensitivity to Tigecycline while they showed various degrees of drug tolerance to 11 drugs such as erythromycin , vancomycin and Oxacillin. The yeast-like fungi (candida albicans) had a high sensitivity to 5 antifungal drugs such as amphotericin B and Itraconazole.
Keywords:cows  endometris  pathogenic bacteria  isolating identification  drug sensitivity testing  drug tolerance
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