| 桑树组织培养物中1-脱氧野尻霉素的产生规律初探 |
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| 引用本文： | 邢辉,李勇,张金芳,王洪利,冀宪领,段祖安.桑树组织培养物中1-脱氧野尻霉素的产生规律初探[J].蚕业科学,2008,34(2):322-326. |
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| 作者姓名： | 邢辉 李勇 张金芳 王洪利 冀宪领 段祖安 |
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| 作者单位： | 山东农业大学林学院,山东,泰安,271018;山东农业大学林学院,山东,泰安,271018;山东农业大学林学院,山东,泰安,271018;山东农业大学林学院,山东,泰安,271018;山东农业大学林学院,山东,泰安,271018;山东农业大学林学院,山东,泰安,271018 |
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| 摘 要： | 为探讨桑树合成1-脱氧野尻霉素(1-DNJ)的代谢途径,采用反相高效液相色谱(RP-HPLC)分析法,研究了桑树品种大10的组织培养物中1-脱氧野尻霉素的产生规律。结果表明:桑树几种组织培养物中1-DNJ的质量分数在无菌苗叶片中为0.309604%,无菌苗中为0.158516%,再生芽中为0.318756%,再生苗中为0.168672%;桑树绿色愈伤组织、疏松愈伤组织、悬浮培养细胞、悬浮细胞诱导愈伤组织中不含1-DNJ。推测1-DNJ产生于由愈伤组织分化为再生芽的阶段。
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| 关 键 词： | 桑树 1-脱氧野尻霉素 组织培养物 |
Research on Production Regularity of 1-deoxynojirimycin in Mulberry Tissue Cultures |
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| XING Hui,LI Yong,ZHANG Jin-Fang,WANG Hong-Li,JI Xian-Ling,DUAN Zu-An.Research on Production Regularity of 1-deoxynojirimycin in Mulberry Tissue Cultures[J].Acta Sericologica Sinica,2008,34(2):322-326. |
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| Authors: | XING Hui LI Yong ZHANG Jin-Fang WANG Hong-Li JI Xian-Ling DUAN Zu-An |
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| Abstract: | To investigate the metabolic pathway of 1-deoxynojirimycin (1-DNJ) in the mulberry, the production regularity of 1-DNJ in the tissue cultures of mulberry Da 10 variety was investigated using reversed phase high performance liquid chromatography method. The result showed that the content of the 1-DNJ was 0.309 604% in aseptic plant leaves, 0.158 516% in aseptic seedling, 0.318 756% in regeneration buds, and 0.168 672% in regeneration plant, respectively. There was no 1-DNJ in green callus, loose callus, suspension cells and induced callus from suspension cells, suggested that the 1-DNJ was produced in the phase of callus differentiation to regeneration buds. |
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| Keywords: | Morus L 1-deoxynojirimycin Tissue cultures |
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