Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA) |
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| Authors: | Lau Lok-Ting Reid Scott M King Donald P Lau Anson Ming-Fung Shaw Andrew E Ferris Nigel P Yu Albert Cheung-Hoi |
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| Institution: | Hai Kang Life Corporation Limited, 8/F Hang Tung Resources Centre, 18 A-Kung Ngam Village Road, Shau Kei Wan, Hong Kong SAR, China. |
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| Abstract: | A study was conducted to evaluate the performance of a nucleic acid sequence-based amplification (NASBA) assay for the detection of foot-and-mouth disease virus (FMDV). Two detection methods: NASBA-electrochemiluminescence (NASBA-ECL) and a newly developed NASBA-enzyme-linked oligonucleotide capture (NASBA-EOC) were evaluated. The diagnostic sensitivity of these assays was compared with other laboratory-based methods using 200 clinical samples collected from different regions of the world. Assay specificity was also assessed using samples (n=43) of other viruses that cause vesicular disease in livestock and genetic relatives of FMDV. Concordant results were generated for 174/200 (87.0%) of suspect FMD samples between NASBA-ECL and real-time RT-PCR. In comparison with the virus isolation (VI) data, the sensitivity of the NASBA-ECL assay was 92.9%, which was almost identical to that of the real-time RT-PCR (92.4%) for the same set of samples. There was broad agreement between the results of the NASBA-ECL and the simpler NASBA-EOC detection method for 97.1% of samples. In conclusion, this study provides further data to support the use of NASBA as a rapid and sensitive diagnostic method for the detection and surveillance of FMDV. |
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