| 金荞麦叶发酵茶抗小鼠H22肿瘤的作用及机制研究 |
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| 引用本文： | 黄小燕,黄莎,陈庆富.金荞麦叶发酵茶抗小鼠H22肿瘤的作用及机制研究[J].安徽农业大学学报,2015,42(6):854-859. |
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| 作者姓名： | 黄小燕 黄莎 陈庆富 |
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| 作者单位： | 1. 贵州师范大学生命科学学院,贵阳,550001;2. 贵州师范大学荞麦产业技术研究中心,贵州省荞麦工程技术研究中心,贵阳550001 |
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| 基金项目： | 贵州省科技厅国际合作项目（黔科合外G字[2013]7021 ）, 国家燕麦荞麦现代农业产业技术体系专项资金(CARS-08-A4), 国家自然科学基金项目(31471562, 31171609), 贵州省农业攻关项目(黔科合NY字[2010]3094), 贵州省科技厅科学技术基金项目（黔科合J字[2012]2268）和贵州省高层次创新型人才培养计划 (黔科合人才[2015]4020号)共同资助。 |
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| 摘 要： | 为探究金荞麦叶发酵茶抑制肿瘤的作用及其机制,以移植H22荷瘤小鼠为模型,设置空白组、金荞麦叶发酵茶低中高剂量组(1.25、2.50和5.00 g·kg-1·d-1)灌胃,阳性药5-FU组,检测其抑瘤效果及对H22小鼠生存时间的影响。以荷瘤小鼠血清中SOD和MDA含量检测评价金荞麦叶发酵茶的抗氧化能力,以耳肿胀度、鸡红细胞(CRBC)致敏小鼠血清溶血素水平、碳廓清能力及小鼠免疫器官的重量评价免疫功能的调节能力。结果发现,金荞麦叶发酵茶高剂量能够明显抑制小鼠H22实体瘤生长(P0.05),其抑瘤率为36.3%;SOD活力与模型组比较显著提高(P0.05),而低剂量组、中剂量组和高剂量组MDA含量较模型组均下降,但与模型组比无明显差异。发酵茶能显著提高正常小鼠DTH反应所致的耳廓肿胀度(P0.01);可提高CRBC致敏小鼠的血清溶血素含量,且显著加强小鼠单核巨噬细胞的吞噬能力,加快碳粒的清除,而对正常小鼠免疫器官的影响比较复杂。可见,金荞麦叶发酵茶通过显著增强小鼠的免疫功能,提高SOD的活性,清除自由基,减轻活性氧带来的损伤,从而起到抗肿瘤作用。
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| 关 键 词： | 金荞麦叶发酵茶 H22肿瘤小鼠 抗肿瘤 迟发型变态反应 溶血素 碳粒廓清 SOD MDA |
Antineoplastic function and mechanism of gold buckwheat leaf fermented tea in the mice of H22 tumor |
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| HUANG Xiaoyan,HUANG Sha and CHEN Qingfu.Antineoplastic function and mechanism of gold buckwheat leaf fermented tea in the mice of H22 tumor[J].Journal of Anhui Agricultural University,2015,42(6):854-859. |
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| Authors: | HUANG Xiaoyan HUANG Sha and CHEN Qingfu |
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| Institution: | School of Life Science, Guizhou Normal University, Guiyang 550001,School of Life Science, Guizhou Normal University, Guiyang 550001 and Research Center of Buckwheat Industry Technology, Guizhou Normal University, Buckwheat Engineering Center in Guizhou Province, Guiyang 550001 |
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| Abstract: | hemolysin level of CRBC sensitized mice and significantly enhanced the ability of mononuclear phagocytic macrophages in mice, as well as accelerated the clearance of carbon particles. The impact of the gold buckwheat fermented tea on immune organs in mice is complicated. In conclusion, the gold buckwheat fermented tea can deploy antineoplastic function in mice by enhancing the immune function, augmenting SOD activity, clearing free radicals and alleviating lesions generated by reactive oxygen. |
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