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| 铁皮石斛可溶性糖含量与蔗糖代谢酶活性的相关性研究 | |
| 引用本文： | 杨骏,孟衡玲,杨生超,张薇,查应洪,文国松.铁皮石斛可溶性糖含量与蔗糖代谢酶活性的相关性研究[J].西部林业科学,2012,41(2):62-67. |
| 作者姓名： | 杨骏 孟衡玲 杨生超 张薇 查应洪 文国松 |
| 作者单位： | 1. 云南农业大学/云南省优势中药材规范化种植工程研究中心,云南昆明,650201 2. 红河学院云南省高校农作物优质高教栽培与安全控制重点实验室,云南蒙自,661100 3. 云南红河群鑫石斛种植有限公司,云南屏边,661200 |
| 基金项目： | 国家支撑计划项目(2011BAI13B02-5)资助 |
| 摘 要： | 通过对F型及H型铁皮石斛不同时期可溶性糖含量与蔗糖代谢酶活性的测定,并对其相关性进行分析,分析了可溶性糖的合成与蔗糖代谢酶活性间的关系。结果表明,移栽后F型和H型铁皮石斛叶片中蔗糖磷酸合成酶的活性与季节性变化趋势一致,但F型较H型高;蔗糖降解酶与可溶性糖含量及其还原糖间存在相关关系,达到显著水平。对可溶性糖和相关因子进行偏相关分析和通径分析可知,还原糖的直接通径系数最大为1.015,酸性转化酶的直接通径系数为负值(-0.356),与可溶性糖呈负相关关系,4个因子对可溶性糖含量的直接作用大小顺序为还原糖>酸性转化酶>中性转化酶>蔗糖。 |
| 关 键 词： | 铁皮石斛 可溶性糖 蔗糖代谢酶 相关性分析 |
Correlation between Soluble Polysaacharide and Sucrose Metabolic Enzymes in Dendrobium officinale |
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| YANG Jun , MENG Heng-ling , YANG Sheng-chao , ZHANG Wei , ZHA Ying-hong , WEN Guo-song.Correlation between Soluble Polysaacharide and Sucrose Metabolic Enzymes in Dendrobium officinale[J].Journal of West China Forestry Science,2012,41(2):62-67. | |
| Authors: | YANG Jun MENG Heng-ling YANG Sheng-chao ZHANG Wei ZHA Ying-hong WEN Guo-song |
| Institution: | 1(1.Yunnan Agricultural University/Yunnan Province Research Center on Good Agricultural Practice for Dominant Chinese Medicinal Materials,Kunming Yunnan 650201,P.R.China;2.The Key Laboratory of High Quality Crops Cultivation and Safety Control,Honghe University,Mengzi Yunnan 661100,P.R.China;3.Yunnan Honghe Qunxin Dendrobium Planting Co.Ltd.,Pingbian Yunnan 661200,P.R.China) |
| Abstract: | Taking F type and H type of Dendrobium officinale of different growth stage as materials,the soluble polysaacharide and sucrose metabolic enzymes were determined.The correlation between these two indexes were analyzed.The results indicated that the change of the activity of sucrose phosphate synthase in F type and H type Dendrobium officinale showed the same trend,the SPS activity of F type was higher than that of H type.There was significant correlation between sucrose degrading enzymes and the contents of soluble sugar and reducing sugar.Partial correlation analysis and path analysis showed the maximum direct path coefficient of reducing sugar was 1.015,the direct path coefficient of acid invertase was-0.356,with negative correlation with soluble sugar.The effects of studied four indexes on the content of soluble sugar decreased in the order of reducing sugar,acid invertase,neutral invertase and sucrose. |
| Keywords: | Dendrobium officinale soluble sugar sucrose metabolic enzyme correlation analysis |
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