| 黏质沙雷氏菌诱导的黄喉拟水龟SMART cDNA文库构建及相关基因的鉴定 |
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| 引用本文： | 赵 密,朱新平,史 燕,高明英.黏质沙雷氏菌诱导的黄喉拟水龟SMART cDNA文库构建及相关基因的鉴定[J].华中农业大学学报,2011,30(1):99-104. |
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| 作者姓名： | 赵 密 朱新平 史 燕 高明英 |
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| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,广州,510380;上海海洋大学水产与生命学院,上海,200090
2. 中国水产科学研究院珠江水产研究所,广州,510380 |
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| 基金项目： | 国家重点基础研究发展计划项目，广东省科技兴渔项目 |
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| 摘 要： |
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| 关 键 词： | 黄喉拟水龟 黏质沙雷氏菌 SMART全长cDNA文库 基因鉴定 |
| 收稿时间： | 2010/4/17 0:00:00 |
Construction of a smart cDNA library of Asian yellow pond turtle stimulated with Serratia marcescens and identification of related genes |
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| ZHAO Mi,ZHU Xin-ping,SHI Yan,GAO Ming-ying.Construction of a smart cDNA library of Asian yellow pond turtle stimulated with Serratia marcescens and identification of related genes[J].Journal of Huazhong Agricultural University,2011,30(1):99-104. |
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| Authors: | ZHAO Mi ZHU Xin-ping SHI Yan GAO Ming-ying |
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| Institution: | ZHAO Mi1,2 ZHU Xin-ping1,2 SHI Yan1 GAO Ming-ying1,21.Pearl River Fisheries Research Institute,Chinese of Fishery Sciences,Guangzhou 510380,China,2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306 |
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| Abstract: | To understand anti-infectious response to bacteria in the Asian yellow pond turtle(Mauremys mutica),a full length cDNA library was constructed for it by SMART technique experimentally infected with Serratia marcescens.Firstly,the double-strand cDNA was synthesized using SMARTTM PCR cDNA systhesis kit.Second,the ds cDNA was separated into two parts based on the size distribution of amplified ds cDNA by agarose gel size fractionation.The part shorter than 500 bp was discarded and the other one longer than 500... |
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| Keywords: | Asian yellow pond turtle Serratia marcescens SMART cDNA library gene identification |
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