Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm |
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| Authors: | Jiaxi Wang Huiji Liu Wenqiang Min Jingo Tong Min Guan Yuzhang Han Luojun Gong Zhen Huang Jie Ren Jinping Zhang Hongping Zheng |
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| Institution: | (1) Hubei Province Fisheries Research Institute, 116 Donghu Road, Wuchang, 430071, Hubei, P.R. China;(2) Academia Sinica, Institute of Hydrobiology, Wuhan, 430072, P.R. China;(3) Zhaoqing Goldeatech Co. Ltd, Zhaoqing, 526200, P.R. China |
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| Abstract: | Effects of the starting time and duration of cold shock, as well as the source of heterogenic sperm on the percentage of viable
gynogenic larvae (PVGL) in tench were studied. The DNA in sperm of red common carp (Cyprinus carpio var singuonensis) was inactivated by ultraviolet radiation prior to use to induce meiotic gynogenetic development in tench. In experiment
1, tench eggs were cold-shocked for 30 min starting at 1, 3, 5, 7, 10 and 15 min post activation. In experiment 2, cold shock
began 5 min after activation and lasted for 10, 20, 30, 40, 50 min, respectively. Each experiment was run in triplicate using
3 tench females, and one group not treated with cold shock was included in each experiment to serve as a control group. In
experiment 3, sperm of bigmouth buffalo (Ictiobus cyprinellus), red common carp or grass carp (Ctenopharyngodon idella) was used to induce gynogenesis in tench with a 20-min cold shock starting 5 min post activation. The results showed that
PVGL from the control group was very low (0.11–0.47%). In experiment 1, the highest average PVGL (9.60%) was observed when
cold shock treatment was applied 5 min post activation. When cold shock treatment was started 5 min post activation, duration
of cold shock affected PVGL. Cold shock lasting 20 min resulted in the highest average PVGL (12. 57%) among the selected duration
of cold shock studied in experiment 2. The average PVGL was 2.3, 8.6, and 9.3%, respectively, for eggs induced by sperm of
bigmouth buffalo, red common carp and grass carp. Average PVGL was significantly lower for eggs induced by sperm of bigmouth
buffalo, compared with that for eggs induced by sperm of the other two species. However, average PVGL were similar for eggs
induced by sperm of red common carp and grass carp. In summary, the optimal conditions for gynogenesis in tench include the
use of irradiated sperm of grass carp to activate the eggs and cold shock of 20 min starting 5 min post activation. Since
female tench grow much faster to a larger size than male tench, gynogenesis of tench holds great potential for production
enhancement. |
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| Keywords: | Heterogenic sperm Meiotic gynogenesis Tinca tinca |
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