| 耐辐射球菌Rsr增强大肠杆菌抗逆性的研究 |
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| 引用本文： | 杨明坤,张颖,侯晓光,郝艳华,张维,陈明.耐辐射球菌Rsr增强大肠杆菌抗逆性的研究[J].核农学报,2011,25(1):53-56. |
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| 作者姓名： | 杨明坤 张颖 侯晓光 郝艳华 张维 陈明 |
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| 作者单位： | 1. 中国农业科学院生物技术研究所,北京,100081
2. 中国农业科学院生物技术研究所,北京,100081;中国农业科学院郑州果树研究所,河南郑州,450009 |
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| 基金项目： | 国家高技术研究发展计划项目，国家自然科学基金，农业部公益性行业科研专项 |
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| 摘 要： | 耐辐射球菌(Deinococcus radiodurans)Rsr蛋白(DR_1262编码)是一种对辐射抗性起重要作用的RNA结合蛋白.本研究将完整的rsr基因连接到带有groESL启动子的pMD-GroE载体上,构建了Rsr表达菌株.以合空载质粒pMD-GroE的大肠杆菌为对照,发现Rsr的表达提高了大肠杆菌的UV辐...
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| 关 键 词： | 耐辐射球菌 大肠杆菌 Rsr 高盐 海藻糖 |
RADIORESISTANT BACTERIUM Deinococcus radiodurans Rsr PROTEIN ENHANCES ABIOTIC STRESS TOLERANCE OF Escherichia coli |
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| YANG Ming-kun,ZHANG Ying,HOU Xiao-guang,HAO Yan-hua,ZHANG Wei,CHEN Ming.RADIORESISTANT BACTERIUM Deinococcus radiodurans Rsr PROTEIN ENHANCES ABIOTIC STRESS TOLERANCE OF Escherichia coli[J].Acta Agriculturae Nucleatae Sinica,2011,25(1):53-56. |
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| Authors: | YANG Ming-kun ZHANG Ying HOU Xiao-guang HAO Yan-hua ZHANG Wei CHEN Ming |
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| Institution: | Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing ;
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences，Zhengzhou, Henan |
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| Abstract: | The Deinococcus radiodurans Rsr protein (encoded by DR_1262),an RNA\|binding protein contributes to radiation resistance. To study the effect of Rsr in Escherichia coli, we compared the abiotic stress response in an E. coli control strain carrying only the pMD-GroE plasmid and a transformant strain expressing Rsr under control of the groESL promoter. The results indicated that D. radiodurans Rsr not only improved the resistance of E. coli to ultraviolet irradiation, but also confered enhanced temperature and salt tolerance in E. coli. Quantitative real-Time PCR analysis showed that the expression of trehalose synthase genes was upregulated under salt stress in the Rsr-expressing strain, suggesting that the accumulation of high levels of the trehalose leads to the improved salt tolerance. |
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| Keywords: | Rsr |
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