| 连翘酯苷A对内毒素致鸡脾淋巴细胞白介素-17的影响 |
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| 引用本文： | 程广东,张强,岳丽红,王长平,陶冶,任重娟,汤方强,李艳华.连翘酯苷A对内毒素致鸡脾淋巴细胞白介素-17的影响[J].安徽农业科学,2017,45(34):106-108. |
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| 作者姓名： | 程广东 张强 岳丽红 王长平 陶冶 任重娟 汤方强 李艳华 |
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| 作者单位： | 佳木斯大学生命科学学院,黑龙江佳木斯154007;东北农业大学动物医学学院,黑龙江哈尔滨150030;佳木斯大学畜牧兽医科学研究所,黑龙江佳木斯154007;佳木斯大学公共卫生学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;东北农业大学动物医学学院,黑龙江哈尔滨,150030 |
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| 基金项目： | 黑龙江省自然科学基金面上项目，佳木斯大学校级青年科学基金项目，佳木斯大学研究生科技创新项目 |
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| 摘 要： | 目的]研究内毒素和连翘酯苷A对鸡脾淋巴细胞中白介素-17(IL-17)水平的影响。方法]将1日龄雏鸡正常饲养至50日龄时,心脏采血处死,表面消毒后,剖检用脾淋巴细胞分离液分离脾淋巴细胞,将脾淋巴细胞分为20个组,具体为4个剂量组×5个时间点。4个剂量组分别为:对照组、LPS组(5μg/m L)、连翘酯苷A预防低剂量组(200μg/m L连翘酯苷A+5μg/m L LPS)、连翘酯苷A预防高剂量组(400μg/m L连翘酯苷A+5μg/m L LPS)。5个时间点分别为0、6、12、24、48 h。通过采用ELISA和Real time-PCR方法检测脾脏淋巴细胞中IL-17水平。结果]ELISA结果显示,与正常组相比,LPS组脾脏淋巴细胞中炎症因子IL-17含量升高;与LPS组比较,连翘酯苷A组脾脏淋巴细胞中上述炎症因子含量下降,表明脾脏中炎症因子的变化呈正相关性,通过抑制LPS诱导的鸡脾脏中上述炎症因子的升高,减轻炎症反应。Real time-PCR结果显示,与正常组相比,LPS组脾脏淋巴细胞中炎症因子IL-17 mRNA表达量升高;与LPS组比较,连翘酯苷A组预防组脾脏淋巴细胞中上述炎症因子表达量下降,变化呈相关性,试验表明连翘酯苷A可以通过转录水平抑制LPS诱导的鸡脾脏淋巴细胞中上述炎症因子表达量的升高,减轻炎症反应。结论]连翘酯苷A抑制鸡脾脏淋巴细胞中IL-17水平,可起到抗炎功能。
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| 关 键 词： | 连翘酯苷A 内毒素 淋巴细胞 白介素-17 |
Effect of Forsythiaside A on IL-17 Level in Spleen Lymphocyte of Chickens Induced By Lipopolysaccharide |
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| Abstract: | Objective] Effect of Forsythiaside A on IL-17 level in spleen lymphocyte of chickens induced by lipopolysaccharide was studied.Method] Day-old chickens were fed normally for 50 days,chickens were killed,collecting the spleen sterilely,by lymphotocyte separation medium,spleen lymphocytes were divided into 20 groups(4 doses × five time points).Four groups included control group,LPS injection group (5 μg/mL),Forsythiaside A low dose treatment group (Forsythiaside A 200 μg/kg + LPS 5 μg/mL),Forsythiaside A high dose treatment group (Forsythiaside A 400 μg/kg + LPS 5 ug/mL).Five time points were:0 h,6 h,12 h,24 h,48 h.Lymphocyte IL-17 level was determined by ELISA and Real time-PCR.Result] ELISA results showed that protein content of IL-17 of spleen lymphocytes increased in LPS groups compared with control group.Forsythiaside A decreased content of above inflammation factor compared with LPS group.Decrease inflammation response showed positive correlation with inflammation factor change of spleen lymphocytes through inhibiting IL-17 of spleen lymphocytes in the chicken by LPS to reduce inflammation reactions.Real time-PCR results showed that IL-17 mRNA expression level of inflammation factor of spleen lymphocytes increased in LPS groups compared with control group.While Forsythiaside A decreased mRNA expression of IL-17.Decreasing inflammation response showed positive correlation with inflammation factor change of spleen lymphocytes through inhibiting inflammation factor of spleen in the chicken by LPS to reduce inflammation reactions.Conclusion] Forsythiaside A perhaps decreased IL-17 level,it had antioxidant and anti-inflammatory function. |
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| Keywords: | Forsythiaside A Lipopolysaccharide Lymphocyte IL-17 |
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