| 中国青凤蝶3个亚种的遗传变异分析 |
| |
| 引用本文： | 杨斯琦,王亚楠,王方方,何娅,靳亚丽.中国青凤蝶3个亚种的遗传变异分析[J].安徽农业科学,2017,45(31):156-159. |
| |
| 作者姓名： | 杨斯琦 王亚楠 王方方 何娅 靳亚丽 |
| |
| 作者单位： | 上海科技馆上海自然博物馆自然史研究中心,上海,200041;中国农业科学院上海兽医研究所农业部动物寄生虫学重点实验室,上海,200241 |
| |
| 基金项目： | 上海市金山区水务局专项“大金山岛猕猴容量调查研究”，上海科技馆种子资金项目 |
| |
| 摘 要： | 目的]探讨中国青凤蝶(Graphium sarpedon Linnaeus)3个亚种的遗传变异。方法]对青凤蝶3个亚种的COⅠ基因和EF-1α基因部分序列进行测定,并对序列的碱基组成、转换颠换数和遗传距离等进行分析。结果]在测得的COⅠ基因(709 bp)和EF-1α(1 064 bp)基因中,有44个变异位点,6个简约信息位点,COⅠ基因A+T平均含量为69.3%,存在较强的含量偏向性,亚种间的遗传距离相差甚小。结论]青凤蝶3个亚种之间没有明显序列差异,仍然适宜采用传统的基于形态学的青凤蝶亚种分类体系。
|
| 关 键 词： | 青凤蝶 COⅠ基因 EF-1α基因 遗传变异 |
Analysis of Genetic Variation in Three Subspecies of Graphium sarpedon Linnaeus from China |
| |
| Abstract: | Objective] To investigate the genetic variation in three subspecies of Graphium sarpedon Linnaeus in China.Method] The CO Ⅰgene and the EF-1 α gene were partially sequenced.The nucleotide composition,the number of transition and transversion,genetic distance of the segment had been analyzed.Result]There were 44 variation sites and 6 parsimony-informative sites in combined sequence of the CO Ⅰgene(709 bp)and EF-1α(1 064 bp) gene in the examined species.The average A + T content of CO Ⅰ gene was 69.3％,which showed strong A + T bias.But genetic distance among subspecies were very small.Conclusion] It has no significant sequence differences between the three subspecies,which is still suitable to use the traditional morphological classification system for Graphium sarpedon subspecies. |
| |
| Keywords: | Graphium sarpedon COⅠ gene EF-1α gene Genetic variation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
