| 黄瓜白粉病抗性遗传分析与连锁标记筛选 |
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| 引用本文： | 聂京涛,潘俊松,何欢乐,司龙亭,蔡润.黄瓜白粉病抗性遗传分析与连锁标记筛选[J].中国蔬菜,2011,1(10):45-49. |
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| 作者姓名： | 聂京涛 潘俊松 何欢乐 司龙亭 蔡润 |
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| 作者单位： | 1.沈阳农业大学园艺学院，辽宁沈阳 110161；2上海交通大学农业与生物学院，上海 200240 |
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| 基金项目： | 上海市科委基础研究重点项目 |
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| 摘 要： | 以黄瓜高感、高抗白粉病自交系M12、M3为亲本组合得到的F2群体和BC1群体为试材，采用苗期接种鉴定，探讨了黄瓜白粉病抗性的遗传规律；结合BSA法和SSR技术，获得了与黄瓜白粉病抗性主效基因连锁的SSR标记。结果表明，供试亲本间白粉病抗性主要受一隐性单基因控制。对F2单株进行SSR分析，鉴定出1个与黄瓜白粉病抗性基因连锁的标记SSR15592，该标记与抗性基因间的遗传距离为7.62 cM。
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| 关 键 词： | 黄瓜 白粉病 抗性基因 遗传分析 SSR |
SSR Inheritance Analysis and Screening for Linked Marker of Powdery Mildew Resistance in Cucumber(Cucumis sativus L.) |
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| NIE Jing-tao,PAN Jun-song,HE Huan-le,SI Long-ting,CAI Run.SSR Inheritance Analysis and Screening for Linked Marker of Powdery Mildew Resistance in Cucumber(Cucumis sativus L.)[J].China Vegetables,2011,1(10):45-49. |
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| Authors: | NIE Jing-tao PAN Jun-song HE Huan-le SI Long-ting CAI Run |
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| Institution: | 1.School of Horticulture, Shenyang Agricultural University, Shenyang 110161, Liaoning, China;2.School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China |
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| Abstract: | In order to accelerate molecular marker assisted breeding process of powdery mildew resistance in cucumber(Cucumis sativus L.),in this paper,high susceptible cucumber inbred line M12,abd high resistant inbred line M3 to powdery mildew were taken as parent and their hybrid,F2 populations and BC1 populations were used as experimental materials.We identified the seedlings inoculated with powdery mildew fungus and probed into the genetic regulation of powdery mildew resistance in cucumber.Combing with BSA metho... |
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| Keywords: | Cucumber Powdery mildew Resistant gene Genetic analysis SSR |
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