| 悬沙胁迫下日本囊对虾仔虾的生长和DNA损伤 |
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| 引用本文： | 李云,沈盎绿,徐兆礼.悬沙胁迫下日本囊对虾仔虾的生长和DNA损伤[J].中国水产科学,2011,18(3):493-499. |
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| 作者姓名： | 李云 沈盎绿 徐兆礼 |
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| 作者单位： | 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业重点开放实验室,上海200090 |
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| 基金项目： | 国家重点基础研究发展计划项目，中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)资助项目 |
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| 摘 要： | 以日本囊对虾(Marsupenaeus japonicus)仔虾为受试生物,研究不同悬沙浓度(0、l0、50、100、500和1 000 mg/L)下仔虾的存活率、增重率、RNA/DNA比率变化和DNA损伤情况,探讨悬沙对仔虾的影响.结果表明,低浓度悬沙(＜500 mg/L)对仔虾的存活率、增重率、RNA/DNA比率和DNA损伤指标无显著影响.高浓度悬沙(≥500 mg/L)为仔虾生长蜕皮提供隐蔽场所,减少自残,但在提高存活率的同时,影响仔虾摄食,表现为仔虾的增重率和RNA/DNA比率与对照组相比显著下降(P＜0.05),且增重率和RNA/DNA比率之间具有显著的正相关性(P＜0.05).在高浓度悬沙组(≥500 mg/L)中,作为DNA损伤指标,彗星细胞拖尾率、尾长和尾矩与对照组相比均显著增加(P＜0.05),但不同的指标灵敏度不同,尾长和尾矩灵敏度较拖尾率更高.结论认为,RNA/DNA比率和DNA损伤指标能够有效评估悬沙对仔虾生长的影响.
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| 关 键 词： | 悬沙 日本囊对虾仔虾 RNA/DNA比率 DNA损伤 |
| 修稿时间： | 2011/5/9 0:00:00 |
Growth and DNA damage of Marsupenaeus japonicus postlarvae stressed by suspended solids |
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| LI Yun,SHENG Anglu,XU Zhaoli.Growth and DNA damage of Marsupenaeus japonicus postlarvae stressed by suspended solids[J].Journal of Fishery Sciences of China,2011,18(3):493-499. |
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| Authors: | LI Yun SHENG Anglu XU Zhaoli |
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| Institution: | LI Yun,SHENG Anglu,XU Zhaoli Key and Open Laboratory of Marine and Estuary Fisheries,Ministry of Agriculture of China,East China Sea Fisheries Re-search Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China |
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| Abstract: | Despite the recognition that increased suspended solids is harmful to aquatic organisms,studies on the responses of them to suspended solids are limited.The present study determined ecotoxicology risk of suspended solids by evaluating the effects of suspended solids on survival rate,weight gain rate,RNA/DNA ratio and DNA damage of Marsupenaeus japonicus postlarvae.The results suggest that suspended solids have little influence on postlarvae in low concentration groups(<500 mg/L),while in high concentration ... |
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| Keywords: | suspended solids Marsupenaeus japonicus postlarvae RNA/DNA ratio DNA damage |
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