| 农药广谱特异性抗体制备技术研究进展 |
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| 引用本文： | 刘媛,余向阳,梁颖,祝金凤,李顺玲,张存政,刘贤进.农药广谱特异性抗体制备技术研究进展[J].江苏农业学报,2009,25(2). |
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| 作者姓名： | 刘媛 余向阳 梁颖 祝金凤 李顺玲 张存政 刘贤进 |
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| 作者单位： | 江苏省农业科学院食品质量安全检测研究所,江苏南京,210014 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 基于抗原-抗体特异性结合反应的农药免疫学检测技术的发展已经日趋成熟,能够帮助实现农药多残留免疫检测的广谱特异性抗体制备技术成为研究热点之一.本文介绍了农药广谱特异性抗体的原理及其常用的3种制备途径,包括选择共性结构半抗原制备抗体,独特型抗体和重组抗体.并对有机磷、拟除虫菊酯、氨基甲酸酯三大类农药的广谱特异性抗体制备及免疫多残留分析技术的研究现状作一综述.
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| 关 键 词： | 农药 广谱特异性抗体 多残留免疫检测 |
Recent Advances in Development of Broad Specificity Antibodies for Pesticides |
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| LIU Yuan,YU Xiang-yang,LIANG Ying,ZHU Jin-feng,LI Shun-ling,ZHANG Cun-zheng,LIU Xian-jin.Recent Advances in Development of Broad Specificity Antibodies for Pesticides[J].Jiangsu Journal of Agricultural Sciences,2009,25(2). |
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| Authors: | LIU Yuan YU Xiang-yang LIANG Ying ZHU Jin-feng LI Shun-ling ZHANG Cun-zheng LIU Xian-jin |
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| Institution: | Institute of Food Quality Safety and Detection;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China |
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| Abstract: | Immunochemical analysis of pesticides based on the specific binding of antigens and antibodies were well established in the past years.The techniques of broad specificity antibodies that contribute to realize multi-residue immunoassay have become one of the main directions of the future development.In this review,the principles of broad specificity antibodies for pesticides are introduced and the main approaches to the antibodies development are discussed.Production of the antibodies and establishment of mu... |
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