| 牛结核病污染场4种检测方法评估 |
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| 引用本文： | 张建东,陈永林,马宏伟,刘天驹,张涛,李钊,赵建东,米吉提·莫合他尔汗,颜成旭,路广计,张喜悦.牛结核病污染场4种检测方法评估[J].中国动物检疫,2020,37(6):90-93. |
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| 作者姓名： | 张建东 陈永林 马宏伟 刘天驹 张涛 李钊 赵建东 米吉提·莫合他尔汗 颜成旭 路广计 张喜悦 |
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| 作者单位： | 山东农业大学动物科技学院;洛阳市动物疫病预防控制中心;河北省动物疫病预防控制中心;石家庄市动物疫病预防控住中心;济源市动物疫病控制中心;新疆维吾尔自治区动物卫生监督所;中国动物卫生与流行病学中心 |
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| 基金项目： | 国家重点研发计划项目（2017YFC1200504）；河北省现代农业产业技术体系奶牛创新团队建设项目（HBCT2018120205） |
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| 摘 要： | 为寻找最适合牛结核病污染场的检测方法,加快牛结核病净化,采用牛结核菌素(PPD)皮内变态反应试验(TST)、比较皮内变态反应试验(SICTT)、牛结核病ELISA-γ-干扰素试验(IFN-γ-ELISA)、牛结核病抗体ELISA试验4种方法,对某结核病污染牛场300头奶牛进行检测,并以TST为参考标准,对不同检测方法的检测结果进行对比分析。结果显示,这4种方法中,IFN-γ-ELISA的阳性检出率最高,与TST相比,差异显著(P<0.05),而其他方法的阳性检出率均低于TST。结果表明,IFN-γ-ELISA方法的检测敏感性较高,在牛结核病污染场净化初期使用,可以尽量避免阳性牛漏检,从而加快牛结核病净化进程。
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| 关 键 词： | 牛结核病 检测方法 评估 污染牛场 奶牛 |
Evaluation on Four Kinds of Detection Methods for Bovine Tuberculosis in Contaminated Farms |
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| Zhang Jiandong,Chen Yonglin,Ma Hongwei,Liu Tianju,Zhang Tao,Li Zhao,Zhao Jiandong,Mijiti Mohetaryhan,Yan Chengxu,Lu Guangji,Zhang Xiyue.Evaluation on Four Kinds of Detection Methods for Bovine Tuberculosis in Contaminated Farms[J].China Journal Of Animal Quarantine,2020,37(6):90-93. |
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| Authors: | Zhang Jiandong Chen Yonglin Ma Hongwei Liu Tianju Zhang Tao Li Zhao Zhao Jiandong Mijiti Mohetaryhan Yan Chengxu Lu Guangji Zhang Xiyue |
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| Institution: | (College of Animal Science and Technology,Shandong Agricultural University,Tai'an,Shandong 271000,China;Luoyang Animal Disease Prevention and Control Center,Luoyang,Henan 471000,China;Hebei Animal Disease Prevention and Control Center,Shijiazhuang,Hebei 050000,China;Shijiazhuang Animal Disease Prevention and Control Center,Shijiazhuang,Hebei 050000,China;Jiyuan Animal Disease Control Center,Jiyuan,Henan 459000,China;Animal Health Supervision Institution of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang 830000,China;China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China) |
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| Abstract: | In order to find out the optimal method for testing bovine tuberculosis in contaminated farms,and to speed up the purification of the disease,300 cows were tested in an contaminated farm respectively by purified protein derivative(PPD)tubculin skin test(TST),single intradermal comparative tuberculin skin test(SICTT),bovine tuberculosis ELISA-γinterferon(IFN-γ-ELISA)and bovine tuberculosis antibody ELISA assay,with the reference of TST,and the results were analyzed as follows:the positive detection rate was highest by IFN-γ-ELISA,which was greatly different from the results detected by TST(P<0.05),for other methods,the positive detection rates were all lower than that by TST.It was concluded that the sensitivity of IFN-γ-ELISA was relatively high,which could avoid any missing in the early stage of purification and could speed up the process of bovine tuberculosis purification. |
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| Keywords: | bovine tuberculosis detection method assessment contaminated cattle farm cow |
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