An oligonucleotide microarray-based assay for identification of phytoplasma 16S ribosomal groups |
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| Authors: | M Nicolaisen A Bertaccini |
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| Institution: | Danish Institute of Agricultural Sciences, Department of Integrated Pest Management, Research Centre Flakkebjerg, DK-4200 Slagelse, Denmark;and;Alma Mater Studiorum –University of Bologna, DiSTA, Plant Pathology, Viale Fanin 42, Bologna, I-40127, Italy |
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| Abstract: | A method using consensus PCR followed by oligonucleotide microarray hybridization was developed for identification of phytoplasma 16Sr ribosomal groups. The array consisted of 21– to 33-nt-long oligonucleotides which were designed to hybridize to individual 16Sr groups. Two oligonucleotides were designed to detect all phytoplasma groups. The array could efficiently identify samples from 16SrI, 16SrII, 16SrIII, 16SrV, 16SrVI, 16SrVII, 16SrIX, 16SrX and 16SrXII ribosomal groups. This microarray-based test represents a rapid method for detection of phytoplasmas in unknown samples and for identification of most 16Sr groups. |
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| Keywords: | consensus PCR disease diagnostics and detection Mollicutes phytoplasmas 16S rRNA |
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