Genetic variation among <Emphasis Type="Italic">Fusarium</Emphasis> isolates from onion,and resistance to <Emphasis Type="Italic">Fusarium</Emphasis> basal rot in related <Emphasis Type="Italic">Allium</Emphasis> species |
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| Authors: | Guillermo A Galván Carole F S Koning-Boucoiran Wim J M Koopman Karin Burger-Meijer Pablo H González Cees Waalwijk Chris Kik Olga E Scholten |
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| Institution: | (1) Plant Research International, Wageningen University and Research Centre, P.O. Box 16, 6700 Wageningen, The Netherlands;(2) Facultad de Agronomía, Universidad de la República, Av. Garzón 780, Montevideo, Uruguay;(3) Koopman Scientific Services, P.O. Box 404, 1600 Enkhuizen, The Netherlands;(4) Centre for Genetic Resources (CGN), Wageningen University and Research Centre, P.O. Box 16, 6700 Wageningen, The Netherlands |
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| Abstract: | The aim of this research was to study levels of resistance to Fusarium basal rot in onion cultivars and related Allium species, by using genetically different Fusarium isolates. In order to select genetically different isolates for disease testing, a collection of 61 Fusarium isolates, 43 of them from onion (Allium cepa), was analysed using amplified fragment length polymorphism (AFLP) markers. Onion isolates were collected in The Netherlands
(15 isolates) and Uruguay (9 isolates), and received from other countries and fungal collections (19 isolates). From these
isolates, 29 were identified as F. oxysporum, 10 as F. proliferatum, whereas the remaining four isolates belonged to F. avenaceum and F. culmorum. The taxonomic status of the species was confirmed by morphological examination, by DNA sequencing of the elongation factor
1-α gene, and by the use of species-specific primers for Fusarium oxysporum, F. proliferatum, and F. culmorum. Within F. oxysporum, isolates clustered in two clades suggesting different origins of F. oxysporum forms pathogenic to onion. These clades were present in each sampled region. Onion and six related Allium species were screened for resistance to Fusarium basal rot using one F. oxysporum isolate from each clade, and one F. proliferatum isolate. High levels of resistance to each isolate were found in Allium fistulosum and A. schoenoprasum accessions, whereas A. pskemense, A. roylei and A. galanthum showed intermediate levels of resistance. Among five A. cepa cultivars, ‘Rossa Savonese’ was also intermediately resistant. Regarding the current feasibility for introgression, A. fistulosum, A. roylei and A. galanthum were identified as potential sources for the transfer of resistance to Fusarium into onion. |
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| Keywords: | Allium cepa A fistulosum A roylei Fusarium oxysporum f sp cepae F proliferatum |
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