| 抗旱植物沙冬青对植物激素反应特性的研究 |
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| 引用本文： | 贾利敏,郭九峰,孙国琴,乔慧蕾,王蕴华.抗旱植物沙冬青对植物激素反应特性的研究[J].内蒙古农业科技,2009(2):25-26. |
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| 作者姓名： | 贾利敏 郭九峰 孙国琴 乔慧蕾 王蕴华 |
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| 作者单位： | 内蒙古农牧业科学院生物中心,内蒙古,呼和浩特,010031 |
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| 基金项目： | 内蒙古自治区科技厅科技计划项目 |
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| 摘 要： | 以沙冬青为材料,选用茎尖、茎段、子叶和下胚轴为外植体进行组织培养,主要研究植物激素6-BA、KT在芽分化中的作用,结果表明 茎尖和茎段在含1mg/L和2mg/L 6-BA的培养基上,形成了玻璃化的丛芽而死亡,在含低浓度6-BA（0.5mg/L,0.1mg/L,0mg/L）的培养基上,能够产生一定比例、无玻璃化生长正常的芽,以6-BA含量为0.5 mg/L分化的芽率较高。在子叶和下胚轴的分化过程中,0.5 mg/L的6-BA,能够提高子叶不定芽的分化频率,作用较KT明显,相反0.5 mg/L KT提高了下胚轴不定芽的分化。
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| 关 键 词： | 沙冬青 组织培养 激素 反应特性 |
The Effect of Phytohormone on Ammopiptanthus Mongolicus, a Drought Resistant Plant |
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| JIA Li-min.The Effect of Phytohormone on Ammopiptanthus Mongolicus, a Drought Resistant Plant[J].Inner Mongolia Agricultural Science and Technology,2009(2):25-26. |
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| Authors: | JIA Li-min |
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| Institution: | Biotechnology Centre;Inner Mongolia Academy of Agriculture and Animal Husbandry;Huhhot 010031;China |
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| Abstract: | The effect of Phytohormone 6-BA and KT on Ammpiptanthus Mongolicus shoot differentiation during tissue culture had been studied with stem apex,stem segment,cotyledon,hypocotyl as explants respectively.Results showed that: shoots can regenerate from stem apex and stem segment when culture in MS medium with 1 and 2mg/L 6-BA,but the shoot leaf then vitrification and died.Normal shoot can formed when cultured in low 6-BA medium(0.5,0.1,0mg/L).The shoot differentiation frequency is better higher in 0.5mg/L 6-BA.... |
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| Keywords: | Ammpiptanthus Mongolicus Tissue culture Phytohormone Reaction characteristics |
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