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| 橡胶树无性系产胶能力株间一致性分析 | |
| 引用本文： | 包杰,张世鑫,史敏晶,田维敏.橡胶树无性系产胶能力株间一致性分析[J].热带作物学报,2014,35(11):2173-2178. |
| 作者姓名： | 包杰 张世鑫 史敏晶 田维敏 |
| 作者单位： | 1. 海南大学农学院,海南海口570228 ;中国热带农业科学院橡胶研究所 农业部橡胶树生物学与遗传资源利用重点实验室 省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室 海南儋州571737 2. 海南大学农学院,海南海口570228 ;海南大学园艺园林学院,海南海口570228 3. 中国热带农业科学院橡胶研究所 农业部橡胶树生物学与遗传资源利用重点实验室 省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室 海南儋州571737 |
| 基金项目： | 现代农业产业技术体系建设专项（No. CARS-34-GW1）；农业部橡胶树生物学重点开放实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理重点实验室 开放课题基金项目（No. KLOF0906）。 |
| 摘 要： | 橡胶树无性系株间产量存在明显差异是导致产量育种选择周期长的主要原因之一。橡胶树的产量主要取决于产胶能力和排胶能力2个因素。树干树皮中的次生乳管数量及乳管细胞内的天然橡胶生物合成效率与产胶能力直接相关。从次生乳管分化和橡胶合成关键因子基因表达2个层面对无性系株间产胶能力的一致性进行分析。结果表明:树皮最内石细胞群以内次生乳管列数与次生韧皮部厚度的比值具有品系特征;在同一品系中,该比值的株间差异小,表现出良好的一致性。8 a割龄大树该比值的株间一致性较3 a幼树差,而在3 a幼树中,树高40 cm处该比值的株间一致性比树高100 cm处更好。因此,以3 a幼树树高40 cm处的该比值作为产量育种早期筛选标记较合适。胶乳中MYC1、HRT2基因的表达量在割胶后上调,并且具有品系特征,但这2个基因的表达量在株间的一致性较差,故在产量育种早期筛选中只能起到辅助作用。 |
| 关 键 词： | 橡胶树无性系 产胶能力 石细胞 次生乳管 MYC1/HRT2基因表达 |
Consistency Analysis of Latex Production Capacity in Rubber Tree Clones |
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| BAO Jie,ZHANG Shixin,SHI Minjing and TIAN Weimin.Consistency Analysis of Latex Production Capacity in Rubber Tree Clones[J].Chinese Journal of Tropical Crops,2014,35(11):2173-2178. | |
| Authors: | BAO Jie ZHANG Shixin SHI Minjing and TIAN Weimin |
| Institution: | 1 College of Agriculture, Hainan University 2 Rubber Research Institute, CATAS/Key Laboratory of Biology and Genetic Resources of Rubber Tree, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops;1 College of Agriculture, Hainan University 3 College of Horticulture and Landscape Architecture;Rubber Research Institute, CATAS/Key Laboratory of Biology and Genetic Resources of Rubber Tree, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops;Rubber Research Institute, CATAS/Key Laboratory of Biology and Genetic Resources of Rubber Tree, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops |
| Abstract: | The obvious difference of latex yield in rubber tree clones is one of the main reasons leading to the long selection cycle in yield breeding. It is well known that the latex yield of rubber tree depends on the ability of latex biosynthesis as well as latex flow, and the latex biosynthesis is determined by two key factors, one of which is the amount of secondary laticifer line in bark and the other is the effeciency of latex regeneration in laticifers. From secondary laticifer differentiation and key gene expression of rubber biosynthesis two aspects, the consistency of latex production capacity in rubber tree clones was studied. The results showed that the ratio of the number of secondary laticifer lines and the thickness of phloem within the innermost stone cell group was a feature closely related with the clones. The standard error of aforementioned ratio much less than the laticifer line number showing well consistency of the ratio in clones. Moreover, the ratio and its standard error changed according to the tree-age and the height of tree. Usually, consistency of the ratio in bark of rubber tree exploited for 8 years was inferior to the 3 years old tree, in which, however, consistency of the ratio in the bark at 40 cm height was better than 100 cm height. So, the ratio of bark at the height 40 cm for 3-year old could be used as an early screening marker for yield breeding. Gene expression of MYC1 and HRT2, possessing clone characteristic, was upregulated after tapping. However, the consistence of expression was low among clones. We proposed that the expression of the two genes may act as an assistant marker in the yield breeding. |
| Keywords: | Rubber tree clones Rubber production capacity Stone cells Secondary laticifer MYC1/HRT2 gene expression |
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