| 抗除草剂基因在粳稻恢复系中的基因枪转化及其在后代中的表达 |
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| 引用本文： | 牛芝霞,朱祯,牛景,吴茜,邹美智,徐鸿林,李艳萍,孙海波.抗除草剂基因在粳稻恢复系中的基因枪转化及其在后代中的表达[J].天津农业科学,2002,8(1):1-6. |
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| 作者姓名： | 牛芝霞 朱祯 牛景 吴茜 邹美智 徐鸿林 李艳萍 孙海波 |
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| 作者单位： | 1. 天津市水稻研究所,天津300112
2. 中国科学院遗传研究所,北京100101 |
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| 基金项目： | 天津市自然科学基金重点项目 (993802811) |
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| 摘 要： | 利用基因枪法把抗除草剂基因bar基因导入粳稻恢复系超优一号,经PCR,PCR-southern杂交分析,证明外源基因已整合到水稻基因组中,且能稳定遗传;田间喷施除草剂抗性检测结果表明,bar基因在转基因恢复系及后代中均表达,表现抗除草剂,在恢复系与不育系所配制的杂交种中,高效表达,表现高抗除草剂;部分转基因植株T1代分离比符合孟德尔显性单基因的遗传规律,即3∶1规律,部分不符合。
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| 关 键 词： | 粳稻恢复系 bar基因 基因枪转化 抗性检测 |
| 文章编号： | 1006-6500(2002)01-0001-06 |
| 修稿时间： | 2001年9月3日 |
Microprojectile-mediated Transformation of Herbicide Resistance Gene in Restoring Line of Japonica Rice and Its Expreesing in Transgenic Plant |
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| Abstract: | The bar gene transformation of young embryo culture cells and its callus of restoring line of japonica rice through microprojectile has been studied. PCR and PCR-southern analysis indicated that bar gene has been integrated into the rice genome and can transmitted to its later generation T1,T2. It is also noticed that bar gene has expressed not only in the transgene restoring line plants but also in its hybrid plants after spraying of herbicide basta, they all have behaved resistance to herbicide basta, especially in the latter, which are made by male sterile line crossing with transgene restoring line. The ratios of separating of T1 plants of part of transgene rice has been discussed, some are accordance with 3∶1 and others not. |
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| Keywords: | restoring line of japonica rice bar gene microprojectile-mediated transformation herbicide resistance |
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