| 巴西橡胶树乳管肌动蛋白细胞骨架与采胶的关系 |
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| 引用本文： | 高政权,孟春晓,吴继林,郝秉中.巴西橡胶树乳管肌动蛋白细胞骨架与采胶的关系[J].热带作物学报,2003,24(3):22-26. |
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| 作者姓名： | 高政权 孟春晓 吴继林 郝秉中 |
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| 作者单位： | 中国热带农业科学院橡胶研究所,农业部热带作物栽培生理学重点开放实验室,海南,儋州,571737 |
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| 摘 要： | 以成龄巴西橡胶树为材料，用免疫印迹技术研究了胶乳中的肌动蛋白，并用异硫氰酸四甲基罗丹明标记的鬼笔环肽荧光探针检查了乳管伤口的肌动蛋白细胞骨架。割胶后收集的胶乳，经超速离心分离的各个组分中，C乳清(胞质溶液)组分含有肌动蛋白。排胶初始期流出的胶乳中肌动蛋白含量多，排胶后期流出的胶乳中肌动蛋白的含量减少。比较了不同割胶强度下收集的胶乳中肌动蛋白的含量，结果表明在强度割胶条件下，胶乳中的肌动蛋白含量较少。用显示肌动蛋白微丝的鬼笔环肽荧光探针检测到，停止排胶时的乳管伤口末端有一团呈现橙红色的荧光物质，表明了肌动蛋白微丝在伤口末端聚积。进行了外施肌动蛋白微丝的解聚剂大环内脂和碘化钾刺激采胶试验，结果表明：施用质量分数1％的碘化钾和饱和浓度的大环内酯水溶液能使胶乳产量增加。这些事实表明，肌动蛋白细胞骨架可能在橡胶树乳管排胶和乳管堵塞中起重要作用。
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| 关 键 词： | 巴西橡胶树 乳管 肌动蛋白 细胞骨架 胶乳产量 |
| 修稿时间： | 2002年12月23 |
Actin Cytoskeleton in Laticiferous Cells of Hevea brasiliensis in Relation to Latex Exploitation |
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| Cao Zhengquan Meng Chunxiao Wu Jilin Hao Bingzhong.Actin Cytoskeleton in Laticiferous Cells of Hevea brasiliensis in Relation to Latex Exploitation[J].Chinese Journal of Tropical Crops,2003,24(3):22-26. |
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| Authors: | Cao Zhengquan Meng Chunxiao Wu Jilin Hao Bingzhong |
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| Abstract: | Latex from adult trees of Hevea brasiliensis Mull. Arg. was tested for actin by using immunobloting with actin antiserum, and the actin cytoskeleton in laticifer wound was investigated by using TRITC Phalloidin fluorescent optical microscopy. Actin was detected in c serum (cytosol) of the latex fractions resulted from ultra-centrifugation. More actin was found in the latex collected immediately after tapping than in the latex collected before latex flow stopped. A comparison between trees with varied tapping intensities showed that less actin appeared in the latex from the trees with more intensive latex exploitation. A mass of TRITC-fluorescent substance was detected in laticifer wounds when latex flow stopped, indicating accumulations of actin cytoskeleton in the wounds. Stimulated exploitation of latex was made with macrolides and KI,which are depolymerizing compounds of actin cytoskeleton. The results showed that application of saturated macrolides solution in water or 10 g/kg KI led to increase in latex yield. The results of the present stndy indicate that actin cytokeleton might play an important role in regulation of latex flow and wound plugging of the laticifers in Hevea brasiliensis. |
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| Keywords: | Hevea brasiliensis laticifer actin cytoskeleton |
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