| 苎麻叶总绿原酸提取工艺研究 |
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| 引用本文： | 范飞军,彭涛,韦名嘉,陈晨,田鑫,刘立军.苎麻叶总绿原酸提取工艺研究[J].安徽农业科学,2011,39(22):13551-13552. |
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| 作者姓名： | 范飞军 彭涛 韦名嘉 陈晨 田鑫 刘立军 |
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| 作者单位： | 1. 华中农业大学植物科技学院,湖北武汉,430070
2. 湖北省嘉鱼县农业局,湖北咸宁,437200 |
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| 基金项目： | 国家麻类产业技术体系(nycytx-19-E12);国家自然科学基金(31000731) |
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| 摘 要： | 目的]采用超声波法提取苎麻叶片绿原酸,设定不同提取条件测定含量并得出最优提取条件。方法]对苎麻头麻叶片烘干处理后,以一定浓度乙醇为提取液,设定乙醇浓度、料液比、温度、pH、提取时间为单因素,采用超声波法提取,并根据单因素实验结果,选取乙醇浓度、料液比、pH三个因素设计正交实验。结果]各单因素对提取效果影响较大,温度设定常温,提取时间2 h最佳;正交结果表明,最优提取条件为40%乙醇、pH=5、料液比1∶16。结论]苎麻叶中含有0.745%的绿原酸,采用超声波法提取效果好,方法简便易行。
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| 关 键 词： | 苎麻叶 绿原酸 超声波法 提取率 |
Research on the Extraction Technology of Chlorogenic Acid from Ramie Leaves |
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| Institution: | FAN Fei-jun et al(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070) |
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| Abstract: | Objective] To extract chlorogenic acid from ramie leaves by ultrasonic method,and set different conditions to detect the extraction yield so as to obtain the optimum extraction condtions.Method] The first quarter ramie leaves were firstly dried,and then extracted by ultrasonic method with ethanol concentration,material-liquid ratio,temperature,pH and extracting time as the single factor,according to the single factor experimental result,three factors of ethanol concentration,material-liquid ratio and pH were chosen to design the orthogonal test.Results] The extraction effect was greatly impacted by each single factor,and it was the best under the room temperature and 2 h extraction time;the orthogonal results showed that,the optimum extraction conditions were 40% ethanol,pH 5 and 1∶16 material-liquid ratio.Conclusion] Ramie leaves contained 0.745% of chlorogenic acid.It is convenient to adopt ultrasonic method with a fine extraction effect. |
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| Keywords: | Ramie leaves Chlorogenic acid Ultrasonic method Extraction rate |
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