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| 杂交粳稻亲本随机扩增多态性DNA（RAPD）分析（研究简报） | |
| 引用本文： | 梁婉琪,潘爱虎,张大兵,黄亚红,曾令珂,袁勤,全立勇,曹黎明,倪林娟.杂交粳稻亲本随机扩增多态性DNA（RAPD）分析（研究简报）[J].上海农业学报,2001,17(2):33-36. |
| 作者姓名： | 梁婉琪 潘爱虎 张大兵 黄亚红 曾令珂 袁勤 全立勇 曹黎明 倪林娟 |
| 作者单位： | 1. 上海市农业科学院农业生物技术研究中心, 2. 南京大学生命科学院生物科学与技术系, 3. 上海市农业科学院作物育种栽培研究所, |
| 摘 要： | 选用60个随机引物对15份杂交粳稻亲本材料进行了RAPD分析，其中10上物的扩增结果具有明显的多态性。在这15份杂交粳稻亲本材料中共扩增得到153条条带，其中的86条为多态性条带。聚类分析结果表明，所有供试验材料可以被明确分区，在此基础上分析了这15个水稻品种的亲缘关系，对田间育种有一定的参考价值。 |
| 关 键 词： | 杂交粳稻 RAPD分析 亲本选育 |
| 文章编号： | 1000-3924(2001)02-33-04 |
| 修稿时间： | 2000年10月15 |
RAPD ANALYSIS OF PARENTS OF HYBRID Japonica RICE |
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| LIANG Wanqi,Pan Aihu,ZHANG DaBing,Huang Yahong,Zeng Lingke,YUAN Qin,Quan Liyong,CAO Liming,Ni Linjuan.RAPD ANALYSIS OF PARENTS OF HYBRID Japonica RICE[J].Acta Agriculturae Shanghai,2001,17(2):33-36. | |
| Authors: | LIANG Wanqi Pan Aihu ZHANG DaBing Huang Yahong Zeng Lingke YUAN Qin Quan Liyong CAO Liming Ni Linjuan |
| Abstract: | of Biological Science and Technology, Nanjing University,Nangjing 210093 ) Abstract Fifteen parents of hybrid japonica rice including five male sterile lines and ten restorer lines were tested for RAPD analysis with sixty random primers. Among the rice lines tested, 153 DNA fragments were generated, of which 86 exhibited polymorphism. Cluster analysis showed that all tested lines could be clearly distinguished. Ten primers used led to the detection of obvious polymorphism among these lines. Based on these results, the evolutionary relationships of these rice lines are analyzed, and these data will provide a valuable reference for field breeding of japonica rice. |
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